論文 - 詳細
| RRC ID | 88479 |
|---|---|
| 著者 | Seki S, Harada S, Sugimoto-Ishige A, Unno M, Sakuma M, Ito S, Yamamoto H, Tanabe A, Matsuoka S, Makino-Okamura C, Ki S, Fukuyama H, Harada M, Tsumagari K, Imami K, Saito T, Kubo M, Suzuki T, Koseki H, Matano T, Miyauchi K. |
| タイトル | TMPRSS2-induced Golgi disruption restricts the incorporation of virus envelope glycoproteins into virions. |
| ジャーナル | EMBO Rep |
| Abstract |
Understanding the relationship between viral proteins and host factors is essential for developing strategies to control virus infections. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infects host cells using angiotensin converting enzyme 2 (ACE2) and transmembrane protease serine 2 (TMPRSS2) as viral receptor and priming protease during the viral entry phase. Here, we report that TMPRSS2 reduces the infectivity of SARS-CoV-2 and HIV-1 during the viral production phase. Treatment of virus-producing cells with a TMPRSS2 inhibitor increases the production of infectious virions. TMPRSS2 enzymatic activity specifically disrupts the trans-Golgi by phosphorylating Golgi stacking proteins through ERK activation, which disturbs virion spike incorporation and structural maturation of the viral envelope glycoproteins, causing lower viral infectivity. We find that SARS-CoV-2 envelope protein (E protein) counteracts this TMPRSS2 activity to rescue SARS-CoV-2-S incorporation. These results demonstrate negative regulation of viral envelope glycoprotein incorporation by TMPRSS2 and reveal that SARS-CoV-2 regulates the Golgi system to create an optimal viral replication environment. |
| 公開日 | 2026-5-19 |
| DOI | 10.1038/s44319-026-00797-2 |
| PII | 10.1038/s44319-026-00797-2 |
| PMID | 42156913 |
| リソース情報 | |
| ヒト・動物細胞 | Jurkat(RCB3052) CACO-2(RCB0988) COS-1(RCB0143) |