論文 - 詳細
| RRC ID | 88486 |
|---|---|
| 著者 | Huang X, Ferris ST, Kim S, Choudhary MNK, Belk JA, Fan C, Qi Y, Sudan R, Xia Y, Desai P, Chen J, Ly N, Shi Q, Bagadia P, Liu T, Guilliams M, Egawa T, Colonna M, Diamond MS, Murphy TL, Satpathy AT, Wang T, Murphy KM. |
| タイトル | Differential usage of transcriptional repressor Zeb2 enhancers distinguishes adult and embryonic hematopoiesis. |
| ジャーナル | Immunity |
| Abstract |
The transcriptional repressor ZEB2 regulates development of many cell fates among somatic, neural, and hematopoietic lineages, but the basis for its requirement in these diverse lineages is unclear. Here, we identified a 400-basepair (bp) region located 165 kilobases (kb) upstream of the Zeb2 transcriptional start site (TSS) that binds the E proteins at several E-box motifs and was active in hematopoietic lineages. Germline deletion of this 400-bp region (Zeb2Δ-165mice) specifically prevented Zeb2 expression in hematopoietic stem cell (HSC)-derived lineages. Zeb2Δ-165 mice lacked development of plasmacytoid dendritic cells (pDCs), monocytes, and B cells. All macrophages in Zeb2Δ-165 mice were exclusively of embryonic origin. Using single-cell chromatin profiling, we identified a second Zeb2 enhancer located at +164-kb that was selectively active in embryonically derived lineages, but not HSC-derived ones. Thus, Zeb2 expression in adult, but not embryonic, hematopoiesis is selectively controlled by the -165-kb Zeb2 enhancer. |
| 巻・号 | 54(7) |
| ページ | 1417-1432.e7 |
| 公開日 | 2021-7-13 |
| DOI | 10.1016/j.immuni.2021.04.015 |
| PII | S1074-7613(21)00177-1 |
| PMID | 34004142 |
| PMC | PMC8282756 |
| MeSH | Animals Cell Differentiation / genetics Cell Lineage / genetics Chromatin / genetics Dendritic Cells / physiology Enhancer Elements, Genetic / genetics* Female Hematopoiesis / genetics* Humans Male Mice Mice, Inbred C57BL Monocytes / physiology Transcription, Genetic / genetics* Zinc Finger E-box Binding Homeobox 2 / genetics* |
| IF | 22.553 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 59 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC09240 |