論文 - 詳細
| RRC ID | 88535 |
|---|---|
| 著者 | Inagaki S, Nakagawa-Tamagawa N, Huynh NZ, Kambe Y, Yagasaki R, Manita S, Fujimoto S, Noda T, Mori M, Teranishi A, Takeshima H, Ishikawa K, Naitou Y, Yokoyama T, Sakamoto M, Hayashi K, Kitamura K, Tagawa Y, Okuda S, Sato TK, Imai T. |
| タイトル | Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo. |
| ジャーナル | Nat Methods |
| Abstract |
Tissue clearing has been widely used for fluorescence imaging of fixed tissues, but its application to live tissues has been limited by toxicity. Here we develop minimally invasive optical clearing media for fluorescence imaging of live mammalian tissues. Light scattering is minimized by adding spherical polymers with low osmolarity to the extracellular medium. A clearing medium containing bovine serum albumin (SeeDB-Live) is compatible with live cells, enabling structural and functional imaging of live tissues, such as spheroids, organoids, acute brain slices and the mouse brains in vivo. SeeDB-Live minimally affects neuronal electrophysiological properties and sensory responses in vivo, and facilitates fluorescence imaging of deep cortical layers in live animals without detectable toxicity to neurons or behavior. We further demonstrate its utility to epifluorescence voltage imaging in acute brain slices and in vivo preparations. Thus, SeeDB-Live expands both the depth and modality range of fluorescence imaging in live mammalian tissues. |
| 巻・号 | 23(4) |
| ページ | 839-853 |
| 公開日 | 2026-4-1 |
| DOI | 10.1038/s41592-026-03023-y |
| PII | 10.1038/s41592-026-03023-y |
| PMID | 41820664 |
| PMC | PMC13076227 |
| MeSH | Animals Brain / cytology Brain / diagnostic imaging Humans Mice Microscopy, Fluorescence / methods Neurons Optical Imaging* / methods Serum Albumin, Bovine / chemistry |
| IF | 30.822 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 597 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 456.0 |
| リソース情報 | |
| 実験動物マウス | RBRC02189 |