Reference - Detail
| RRC ID | 88556 |
|---|---|
| Author | Furuya H, Toda Y, Iwata A, Kanai M, Kato K, Kumagai T, Kageyama T, Tanaka S, Fujimura L, Sakamoto A, Hatano M, Suto A, Suzuki K, Nakajima H. |
| Title | Stage-specific GATA3 induction promotes ILC2 development after lineage commitment. |
| Journal | Nat Commun |
| Abstract |
Group 2 innate lymphoid cells (ILC2s) are a subset of innate lymphocytes that produce type 2 cytokines, including IL-4, IL-5, and IL-13. GATA3 is a critical transcription factor for ILC2 development at multiple stages. However, when and how GATA3 is induced to the levels required for ILC2 development remains unclear. Herein, we identify ILC2-specific GATA3-related tandem super-enhancers (G3SE) that induce high GATA3 in ILC2-committed precursors. G3SE-deficient mice exhibit ILC2 deficiency in the bone marrow, lung, liver, and small intestine with minimal impact on other ILC lineages or Th2 cells. Single-cell RNA-sequencing and subsequent flow cytometry analysis show that GATA3 induction mechanism, which is required for entering the ILC2 stage, is lost in IL-17RB+PD-1- late ILC2-committed precursor stage in G3SE-deficient mice. Cnot6l, part of the CCR4-NOT deadenylase complex, is a possible GATA3 target during ILC2 development. Our findings implicate a stage-specific regulatory mechanism for GATA3 expression during ILC2 development. |
| Volume | 15(1) |
| Pages | 5610 |
| Published | 2024-7-5 |
| DOI | 10.1038/s41467-024-49881-y |
| PII | 10.1038/s41467-024-49881-y |
| PMID | 38969652 |
| PMC | PMC11226602 |
| MeSH | Animals Cell Differentiation / immunology Cell Lineage* Enhancer Elements, Genetic / genetics GATA3 Transcription Factor* / genetics GATA3 Transcription Factor* / metabolism Immunity, Innate* Lymphocytes* / cytology Lymphocytes* / immunology Lymphocytes* / metabolism Mice Mice, Inbred C57BL Mice, Knockout Single-Cell Analysis Th2 Cells / immunology |
| IF | 12.121 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | News |
| Total number of mentions | 19 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Mice | RBRC00144 |