| RRC ID |
88654
|
| 著者 |
Laurent JM, Steinacher M, Kan A, Ritter M, Leutert M, Bienz S, Häberlin D, Kumar N, Studart AR.
|
| タイトル |
Genetic Impacts on the Structure and Mechanics of Cellulose Made by Bacteria.
|
| ジャーナル |
Adv Sci (Weinh)
|
| Abstract |
The synthesis of cellulose pellicles by bacteria offers an enticing strategy for the biofabrication of sustainable materials and biomedical devices. To leverage this potential, bacterial strains that overproduce cellulose are identified through directed evolution technology. While cellulose overproduction is linked with a specific genetic mutation, the effect of such mutation on the intracellular protein landscape and on the structure and mechanical properties of the cellulose pellicles is not yet understood. Here, the proteome of bacteria evolved to overproduce cellulose is studied and its effect on the structure and mechanics of the resulting cellulose pellicles is investigated. Proteomic analysis reveals that the protein landscape of the evolved bacteria shows pronounced differences from that of native microorganisms. Thanks to concerted changes in the proteome, the evolved bacteria can generate cellulose pellicles with exquisite structure and improved mechanical properties for applications in textiles, packaging, and medical implants.
|
| 巻・号 |
12(33)
|
| ページ |
e05075
|
| 公開日 |
2025-9-1
|
| DOI |
10.1002/advs.202505075
|
| PMID |
40470676
|
| PMC |
PMC12412545
|
| MeSH |
Bacteria* / genetics
Bacteria* / metabolism
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Cellulose* / biosynthesis
Cellulose* / chemistry
Cellulose* / genetics
Cellulose* / metabolism
Proteome / genetics
Proteomics / methods
|
| リソース情報 |
| 一般微生物 |
JCM9730 |
