論文 - 詳細
| RRC ID | 88657 |
|---|---|
| 著者 | Suenari Y, Matsubara T, Hiroshima Y, Pahlevi MR, Shimada H, Ochi Y, Okada N, Sato M, Fujii H, Sumitomo T, Kishimoto H, Murakami A. |
| タイトル | Characterization of Lachnoanaerobaculum sanguinis sp. nov., isolated from a blood culture of an acute myeloid leukemia patient with chemotherapy-related bacteremia. |
| ジャーナル | Anaerobe |
| Abstract |
OBJECTIVE:This study was to characterize and determine the classification of the novel strain TM49T, isolated from a blood culture of a patient who developed bacteremia while receiving high-dose cytarabine chemotherapy for acute myeloid leukemia. METHODS:The novel strain TM49T was characterized at the species level through a polyphasic taxonomic analysis, incorporating morphological, physiological, biochemical, phylogenetic, and genome sequence analyses. RESULTS:16S rRNA gene sequence analysis revealed that the strain belonged to the genus Lachnoanaerobaculum. The 16S rRNA gene sequence of strain TM49T was observed to be most similar to that of "Lachnoanaerobaculum gingivalis" strain ChDC B114T (99.2 %). Strain TM49T formed circular, glossy, slightly milky colonies with an undulate margin on BD™ Columbia Agar containing 5 % sheep blood after 48 h at 37 °C, exhibiting less spreading compared to species of the genus Lachnoanaerobaculum. The major fatty acids of strain TM49T were C16:0, C14:0, C18:1 ω9c DMA, and C18:1 ω7c DMA. The draft genome sequence of strain TM49T was 2,821,554 bp in length, with a G + C content of 36.4. Digital DNA-DNA hybridization values between strain TM49T and "L. gingivalis" ChDC B114T, L. umeaense CD3: 22T, L. orale N1T, and L. saburreum CCUG 28089T were 52.2 %, 29.7 %, 26.2 %, and 24.5 %, respectively. The Orthologous Average Nucleotide Identity values between strain TM49T and "L. gingivalis" ChDC B114T, L. umeaense CD3: 22T, L. orale N1T, and L. saburreum CCUG 28089T were 93.1 %, 84.9 %, 83.2 %, and 81.5 %, respectively. CONCLUSION:Strain TM49T (JCM 36186T = DSM 116945T) was found to be distinct from previously described species of the genus Lachnoanaerobaculum. Therefore, it is proposed as a new species, Lachnoanaerobaculum sanguinis sp. Nov., with strain TM49T as the type strain (= JCM 36186T = DSM 116945T). |
| 巻・号 | 93 |
| ページ | 102961 |
| 公開日 | 2025-6-1 |
| DOI | 10.1016/j.anaerobe.2025.102961 |
| PII | S1075-9964(25)00024-1 |
| PMID | 40233837 |
| MeSH | Bacteremia* / microbiology Bacterial Typing Techniques Base Composition Blood Culture Cytarabine / adverse effects Cytarabine / therapeutic use DNA, Bacterial / chemistry DNA, Bacterial / genetics Eubacteriales* / classification Eubacteriales* / genetics Eubacteriales* / isolation & purification Fatty Acids / analysis Genome, Bacterial Humans Leukemia, Myeloid, Acute* / complications Leukemia, Myeloid, Acute* / drug therapy Male Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA |
| リソース情報 | |
| 一般微生物 | JCM36186 |