| 著者 |
Wang J, Zhu Z, Zhu J, Shen C, Liu J, Peng Z, Yang B, Zhang Y, Fang Z.
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| Abstract |
A Gram-stain-negative, facultatively anaerobic, rod-shaped bacterial strain, namely F4206T, was isolated from the sediments of Beihai Red Beach, China. The optimal temperature, pH, and NaCl concentration of F4206T were 20-40 °C, pH 6.5, and 10%. The digital DNA-DNA hybridization (dDDH), average nucleotide identity (ANI) and average amino acid identity (AAI) values between strain F4206T and its closest relatives ranged from 20.5 to 44.3%, 78.2 to 80.5% and 65.0 to 94.8% respectively, which are well below the established species delineation thresholds of 70%, 95% and 95%. This conclusive genomic evidence for novelty is further supported by phylogenetic analysis, which placed strain F4206T within a distinct lineage closely related to Marinobacter qingdaonensis ASW11-75 T and Marinobacter arenosus CAU 1620 T, with a 16S rRNA gene sequence similarity of 98.22%. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain F4206T formed a distinct lineage within the genus Marinobacter. The digital DNA-DNA hybridization and average nucleotide identity values between strain F4206T and closely related strains were 20.5-44.3% and 78.2-80.5%, much lower than the cutoff values of 70% and 95%, respectively. The dominant respiratory quinone of the strain was ubiquinone-9. The main fatty acids (> 5%) were C16:0, C18:1 ω9c, C16:1 ω9c, summed feature 3, C12:0 3-OH and C12:0. The major polar lipids of strain F4206T were phosphatidylethanolamine, phosphatidylglycerol, aminolipid, and aminophospholipid. The DNA G + C content of F4206T was 58.5%. According to phenotypic, phylogenetic, and chemotaxonomic analysis, strain F4206T was considered as a new species of Marinobacter, with the name of Marinobacter liaoningensis sp. nov. F4206T. The type strain is F4206T (= CGMCC 1.19487 T = KCTC 92054 T).
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