| RRC ID |
88679
|
| Author |
Jayan JN, Lee YM, You YJ, Srinivasan S, Lee SS.
|
| Title |
Robertkochia aurantiaca sp. nov., and Robertkochia flava sp. nov., two potential quinate- and catechol-degrading bacteria isolated from tidal-flat sediments.
|
| Journal |
Antonie Van Leeuwenhoek
|
| Abstract |
Two Gram-negative-strains, 3YJGBD-33T and 3YJGBD-35T were isolated from tidal flat sediment in Daebudo, Republic of Korea. The isolates are non-motile, and long rods. Both strains are oxidase-positive and grew in the range of 15-45 °C (optimum, 40 °C) and pH 7-8 (optimum pH 8.0). Growth was observed in the salinity range of 1-6%, with optimum growth at 4.0% for strain 3YJGBD-33T and 3.0% for strain 3YJGBD-35T. According to the NCBI 16S ribosomal RNA sequences database the strain 3YJGBD-33T and 3YJGBD-35T showed the highest 16S rRNA gene similarity with Robertkochia marina CC-AMO-30DT (97.0% and 98.4%), Robertkochia sediminum 1368T (96.4% and 98.3%) and Robertkochia solimangrovi CL23T (96.0% and 96.5%). The major respiratory quinone is MK-6. The polar lipid profile consists of phosphatidylethanolamine, three aminolipids and two unidentified lipids. The major cellular fatty acids for strains 3YJGBD-33T and 3YJGBD-35T were iso-C15:0, iso-C15:1 G and iso-C17:0 3-OH. The draft genome of strains 3YJGBD-33T and 3YJGBD-35T are 3.4 Mbp and 3.5 Mbp in size with 2993 coding genes, 41 tRNAs and 2935 coding genes, 42 tRNAs, respectively. The polyphasic analysis confirms that strains 3YJGBD-33T and 3YJGBD-35T belong to the genus Robertkochia and represent novel species, for which the names Robertkochia aurantiaca sp. nov. and Robertkochia flava sp. nov. are proposed. The type strains are 3YJGBD-33T (= KCTC 82715T = TBRC 19393T) and 3YJGBD-35T (= KCTC 82716T = JCM 34784T).
|
| Volume |
118(8)
|
| Pages |
105
|
| Published |
2025-7-7
|
| DOI |
10.1007/s10482-025-02102-y
|
| PII |
10.1007/s10482-025-02102-y
|
| PMID |
40622654
|
| MeSH |
Bacterial Typing Techniques
Base Composition
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
Fatty Acids / analysis
Geologic Sediments* / microbiology
Phospholipids / analysis
Phylogeny
RNA, Ribosomal, 16S / genetics
Republic of Korea
Sequence Analysis, DNA
|
| Resource |
| General Microbes |
JCM34784
JCM18552 |
