| RRC ID |
88688
|
| 著者 |
Pitt A, Lienbacher S, Schmidt J, Neumann-Schaal M, Wolf J, Oren A, Reichl S, Hahn MW.
|
| タイトル |
Aquirufa esocilacus sp. nov., Aquirufa originis sp. nov., Aquirufa avitistagni, and Aquirufa echingensis sp. nov. discovered in small freshwater habitats in Austria during a citizen science project.
|
| ジャーナル |
Arch Microbiol
|
| Abstract |
Within a citizen science project, 112 freshwater habitats in Austria were sampled to get bacterial cultures belonging to the genus Aquirufa using a strategy for targeted isolation. We focused on these bacteria because they are widespread and represent typical freshwater bacteria and, furthermore, the typic red pigmentation facilitates preselection. Among the 113 obtained Aquirufa strains were HETE-83DT, KTFRIE-69FT, OSTEICH-129VT and PLAD-142S6KT, originating from small ponds and a creek. Phylogenetic reconstructions with 16S rRNA gene sequences and genome-based analyses with amino acid sequences of 501 core genes showed that all four strains belonged to the A. antheringensis branch of the genus Aquirufa. Calculation of whole-genome average nucleotide identity values and digital DNA-DNA hybridization values revealed that they represent in each case a new species. The genome sizes of the four strains were between 2.5 and 2.8 Mbp and the G + C values were between 41.4 and 41.8%. Like all type strains of the genus Aquirufa, cells were rod-shaped, and liquid cultures and colonies on agar plates were red-pigmented, likely due to carotenoids. All strains except OSTEICH-129VT showed gliding motility on soft agar plates. All strains grew aerobically but only PLAD-142S6KT could grow weakly under anaerobic conditions. We propose here to establish the names Aquirufa esocilacus sp. nov. for strain HETE-83DT (= DSM 118087T = JCM 37094T), Aquirufa originis sp. nov. for KTFRIE-69FT (= DSM 117798T = JCM 37095T), Aquirufa avitistagni for OSTEICH-129VT (= DSM 118088T = JCM 37100T) and Aquirufa echingensis sp. nov. for PLAD-142S6KT (= DSM 117799T = JCM 37096T).
|
| 巻・号 |
207(4)
|
| ページ |
71
|
| 公開日 |
2025-2-25
|
| DOI |
10.1007/s00203-025-04275-6
|
| PII |
10.1007/s00203-025-04275-6
|
| PMID |
39998640
|
| PMC |
PMC11861422
|
| MeSH |
Austria
Bacterial Typing Techniques
Base Composition
Citizen Science
DNA, Bacterial / genetics
Ecosystem
Fresh Water* / microbiology
Genome, Bacterial / genetics
Nucleic Acid Hybridization
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
|
| リソース情報 |
| 一般微生物 |
JCM37094
ﾃ｢ﾂﾂ雨CM37095
ﾃ｢ﾂﾂ雨CM37100
ﾃ｢ﾂﾂ雨CM37096 |
