論文 - 詳細
| RRC ID | 88801 |
|---|---|
| 著者 | Basak C, Ranjan VK, Mondal N, Sarkar J, Ghosh W, Chakraborty R. |
| タイトル | Verticiella alkaliphila sp. nov., An Alkaliphilic, Arsenic-Resistant Bacterium Isolated from the Gut of Lepidocephalichthys guntea. |
| ジャーナル | Indian J Microbiol |
| Abstract |
UNLABELLED:Detailed phenotypic and genotypic characterization was done to establish the taxonomic position of a novel bacterial strain GG226T, frequently been isolated from the gut of Lepidocephalichthys guntea. Strain GG226T a Gram-negative, facultatively anaerobic, rod-shaped, motile, alkaliphilic bacterium. The 16S rRNA gene sequences analysis disclosed that GG226T belongs to genus Verticiella due to its maximum sequence identity with Verticiella sediminum XH089T (98.4%), the sole representative of this genus. A genome BLAST distance phylogeny (GBDP) tree, single nucleotide polymorphism (SNP) tree, and multi locus species tree (MLST) reconfirmed that GG226T shares its closest taxonomic identity with Verticiella sediminum XH089T. The DNA G + C content was estimated to be 67 mol%. When compared with Verticiella sediminum XH089T the DNA-DNA hybridization (dDDH) value was 21.90% and the average nucleotide identity (ANI) value was 80.13%; both of which were sufficiently below the threshold value (≤ 70% and ≤ 95-96%) for new prokaryotic species validation. The dominant fatty acids (> 5% of total fatty acids) were C16:0, summed feature 3 (C16:1ω7c/C16:1ω6c), C17:0 cyclo, and C12:0 3-OH. The strain GG226T has shown significant growth in pH 9 and 10, and noticeable high resistance to sodium arsenate (MIC: > 500 mM). The results of biochemical tests, phenotypic characteristics, genotypic data, and physiological properties could differentiate strain GG226T from its closest taxonomic relative. Hence, characterization based on chemotaxonomic, phenotypic, genomic, and phylogenetic evidence demonstrated that the strain GG226T represents a novel species of the genus Verticiella, for which the name Verticiella alkaliphila sp. nov. (type strain GG226T = MCC 5181T = JCM 36073T) is proposed. SUPPLEMENTARY INFORMATION:The online version contains supplementary material available at 10.1007/s12088-024-01287-6. |
| 巻・号 | 65(2) |
| ページ | 947-954 |
| 公開日 | 2025-6-1 |
| DOI | 10.1007/s12088-024-01287-6 |
| PII | 1287 |
| PMID | 40655345 |
| PMC | PMC12246344 |
| リソース情報 | |
| 一般微生物 | JCM36073 |