論文 - 詳細
| RRC ID | 88950 |
|---|---|
| 著者 | Amano S, Matsumoto M, Morimoto M, Kawamoto H, Takeshita F, Yasui T, Sakagami H. |
| タイトル | Efficacy of toothpaste containing Brazilian green propolis extracts with an optimal kaempferide/betuletol ratio for improving oral microbiota: A randomized, controlled, paired crossover study. |
| ジャーナル | J Ethnopharmacol |
| Abstract |
ETHNOPHARMACOLOGICAL RELEVANCE:Propolis is a resinous substance collected by honeybees from various plant sources and has been used in traditional folk medicine for centuries. Propolis has various biological properties, including antibacterial, antiviral, anti-inflammatory, and anti-tumor properties. The use of propolis in oral health care is attributable to its antimicrobial and anti-inflammatory effects. However, limited evidence exists on the in vivo efficacy of propolis against periodontal pathogens. AIM OF THE STUDY:We aimed to evaluate the efficacy of Brazilian green propolis (BGP)-containing toothpaste for improving the oral environment and define its antibacterial compounds. MATERIALS AND METHODS:Overall, 48 student volunteers aged 18-40 years (24 females and 24 males) were randomly categorized into the BGP and placebo groups. The BGP and placebo groups received toothpaste with and without BGP, respectively. After a baseline assessment, the plaque index (PI) score, gingival index (GI) score, and proportion of periodontal pathogens on the tongue surface were analyzed at 0, 1, and 2 weeks. Antibacterial compounds were identified using liquid-liquid partitioning, high-performance liquid chromatography purification, and nuclear magnetic resonance methods. RESULTS:The concentration of BGP in the toothpaste was set at 0.0347 w/v%. Compared with the placebo group, the BGP group demonstrated a reduction in the PI score (p < 0.05) but not in the GI score, as well as a reduction in Porphyromonas gingivalis (Pg)/Total bacteria (Tb), Fusobacterium nucleatum (Fn)/Tb, and Aggregatibacter actinomycetemcomitans (Aa)/Tb (p < 0.05) but not in Streptococcus salivalius/Tb. Effect sizes for Pg, Fn and Aa were 0.360, 0.556, and 0.164, respectively. The antibacterial compounds of the BGP-containing toothpaste included a mixture of kaempferide/betuletol. CONCLUSIONS:We confirmed the efficacy of propolis toothpaste with an optimal kaempferide/betuletol ratio for improving oral microbiota, thereby suggesting that BGP toothpaste is clinically useful in maintaining oral health and preventing periodontal disease. |
| 巻・号 | 337(Pt 1) |
| ページ | 118762 |
| 公開日 | 2025-1-30 |
| DOI | 10.1016/j.jep.2024.118762 |
| PII | S0378-8741(24)01061-4 |
| PMID | 39214193 |
| MeSH | Adolescent Adult Anti-Bacterial Agents* / administration & dosage Anti-Bacterial Agents* / pharmacology Brazil Cross-Over Studies* Dental Plaque Index Double-Blind Method Female Humans Male Microbiota / drug effects Mouth / microbiology Periodontal Index Propolis* / administration & dosage Propolis* / pharmacology Toothpastes* / pharmacology Young Adult |
| リソース情報 | |
| 一般微生物 | JCM12257 JCM8525 |