Reference - Detail
| RRC ID | 88974 |
|---|---|
| Author | Kishimoto S, Takahashi R, Nagasawa A, Watanabe K. |
| Title | Discovery, Biological Activity, and Biosynthesis of Pinocicolin A, an Antibiotic Isocyanide Metabolite Produced by Penicillium pinophilum. |
| Journal | J Nat Prod |
| Abstract |
We discovered new natural products pinocicolins A (1) and B (2) from Penicillium pinophilum and determined the stereochemistry of these compounds by degradation and derivatization. Compound 1, containing two isocyanide groups, exhibited bacteriostatic activity against Gram-positive bacteria depending upon its copper-chelating activity. The biosynthesis of compound 1 was reconstructed in Aspergillus nidulans by heterologous expression of multidomain isocyanide synthase-nonribosomal peptide synthetase Pp_crmA and efflux pump Pp_crmE. This is the first report of the heterologous production of a fungal diisocyanide natural product, highlighting the multistep catalysis of CrmA family enzymes. |
| Volume | 88(4) |
| Pages | 1068-1074 |
| Published | 2025-4-25 |
| DOI | 10.1021/acs.jnatprod.5c00174 |
| PMID | 40180616 |
| MeSH | Anti-Bacterial Agents* / biosynthesis Anti-Bacterial Agents* / chemistry Anti-Bacterial Agents* / isolation & purification Anti-Bacterial Agents* / pharmacology Aspergillus nidulans / genetics Aspergillus nidulans / metabolism Cyanides* / chemistry Cyanides* / metabolism Cyanides* / pharmacology Gram-Positive Bacteria / drug effects Microbial Sensitivity Tests Molecular Structure Penicillium* / chemistry Penicillium* / metabolism Peptide Synthases / metabolism |
| Resource | |
| General Microbes | JCM23043 |