論文 - 詳細
| RRC ID | 88976 |
|---|---|
| 著者 | Miyata T, Takeichi O, Imai K, Okano M, Inoue S, Yasukawa T, Suzuki Y. |
| タイトル | Reactivation of Epstein-Barr virus by n-butyric acid from Pseudoramibacter alactolyticus induces inflammatory cytokines in periapical granulomas. |
| ジャーナル | J Oral Biosci |
| Abstract |
OBJECTIVES:This study investigates whether latent Epstein-Barr virus (EBV) can be reactivated by n-butyric acid from Pseudoramibacter alactolyticus, and if such reactivation induces expression of interleukin (IL)-1β and IL-6 in periapical granulomas. METHODS:We analyzed periapical granulomas and healthy gingival tissues to detect the presence of EBV and P. alactolyticus. The concentration of n-butyric acid in P. alactolyticus culture supernatants was measured. BZLF-1 luciferase assays were conducted with or without these supernatants. Immunohistochemical detection of ZEBRA-, IL-1β-, and IL-6-expressing cells was performed in the tissue samples. Additionally, mRNA expression levels of BZLF-1, IL-1β, and IL-6 were quantified and statistically analyzed for correlation. The expression of these mRNAs was also measured in Daudi cells treated with or without the culture supernatants. RESULTS:Both EBV and P. alactolyticus were detected in periapical granulomas, but not in healthy tissues. The concentration of n-butyric acid in the culture supernatants was ∼3.58 mmol/L. BZLF-1 luciferase activity in the presence of the culture supernatants was comparable to that of commercially available butyric acid, whereas no activity was detected without the supernatants. Cells expressing ZEBRA co-expressed IL-1β and IL-6. The mRNA levels of IL-1β and IL-6 in periapical granulomas were correlated with BZLF-1 mRNA levels. Daudi cells treated with the culture supernatants expressed BZLF-1, IL-1β, and IL-6 mRNA, while those without the supernatants did not. CONCLUSIONS:The study concludes that EBV can be reactivated by n-butyric acid produced by P. alactolyticus, leading to the induction of IL-1β and IL-6 expression in periapical granulomas. |
| 巻・号 | 67(1) |
| ページ | 100569 |
| 公開日 | 2025-3-1 |
| DOI | 10.1016/j.job.2024.10.001 |
| PII | S1349-0079(24)00203-2 |
| PMID | 39401546 |
| MeSH | Adult Cytokines* / metabolism Female Gingiva / virology Herpesvirus 4, Human* / drug effects Herpesvirus 4, Human* / physiology Humans Interleukin-1beta / metabolism Interleukin-6 / metabolism Male Periapical Granuloma* / metabolism Periapical Granuloma* / microbiology Periapical Granuloma* / virology RNA, Messenger / metabolism Virus Activation* / drug effects |
| リソース情報 | |
| 一般微生物 | JCM6480 |