Reference - Detail
| RRC ID | 88993 |
|---|---|
| Author | Tu Z, Yumoto I. |
| Title | Isolation of a Tissierellaceae Bacterium Exhibiting a High Reduction Potential for Insoluble Indigo Dyes. |
| Journal | Microbes Environ |
| Abstract |
In traditional indigo dyeing, water-insoluble indigo is anaerobically converted into soluble leuco-indigo via microbial reduction in alkaline dye suspensions, allowing its use as a fabric dye. Although various indigo-reducing bacteria have been isolated to date, culture-independent microbial community ana-lyses have suggested that bacteria belonging to uncultured clades also contribute to indigo reduction. Therefore, we aimed to isolate previously overlooked indigo-reducing bacteria using an unconventional culture method. We conducted enrichment cultures and single-colony isolation using a medium supplemented with sukumo, an indigo dye source derived from the composted leaves of indigo-containing plants, as the sole energy, carbon, and nitrogen sources. We isolated a previously uncultured bacterium belonging to the family Tissierellaceae, which had been predicted as a major indigo reducer in various indigo dyeing processes solely based on microbial community ana-lyses. The insoluble indigo-reducing activity of the Tissierellaceae isolate, strain TU-1 was significantly higher than that of known indigo-reducing bacteria. The addition of the culture supernatant of strain TU-1 enhanced the reduction of indigo powder by other indigo-reducing bacteria, with similar stimulatory effects to those of the insoluble electron mediator, anthraquinone. These results indicate that strain TU-1 possesses a high capacity for secreting electron mediators, conferring a significant reduction capacity for insoluble indigo. Further investigations, including the discovery of additional unknown indigo-reducing bacteria and the identification of the mediators they produce, will provide a more detailed understanding of the mechanisms underlying indigo reduction in practical dyeing processes. |
| Volume | 40(3) |
| Published | 2025-1-1 |
| DOI | 10.1264/jsme2.ME24104 |
| PMID | 40653370 |
| PMC | PMC12501876 |
| MeSH | Actinobacteria* / classification Actinobacteria* / genetics Actinobacteria* / isolation & purification Actinobacteria* / metabolism Coloring Agents* / chemistry Coloring Agents* / metabolism DNA, Bacterial / genetics Indigo Carmine* / metabolism Oxidation-Reduction Phylogeny Plant Leaves RNA, Ribosomal, 16S / genetics |
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 1.0 |
| Resource | |
| General Microbes | JCM37169 JCM17250 JCM18529 JCM12662 JCM34140 |