| RRC ID |
89041
|
| Author |
Yamaura K, Takemata N, Kariya M, Osaka A, Ishino S, Yamauchi M, Tamura T, Hamachi I, Takada S, Ishino Y, Atomi H.
|
| Title |
Chromosomal domain formation by archaeal SMC, a roadblock protein, and DNA structure.
|
| Journal |
Nat Commun
|
| Abstract |
In eukaryotes, structural maintenance of chromosomes (SMC) complexes form topologically associating domains (TADs) by extruding DNA loops and being stalled by roadblock proteins. It remains unclear whether a similar mechanism of domain formation exists in prokaryotes. Using high-resolution chromosome conformation capture sequencing, we show that an archaeal homolog of the bacterial Smc-ScpAB complex organizes the genome of Thermococcus kodakarensis into TAD-like domains. We find that TrmBL2, a nucleoid-associated protein that forms a stiff nucleoprotein filament, stalls the T. kodakarensis SMC complex and establishes a boundary at the site-specific recombination site dif. TrmBL2 stalls the SMC complex at tens of additional non-boundary loci with lower efficiency. Intriguingly, the stalling efficiency is correlated with structural properties of underlying DNA sequences. Our study illuminates a eukaryotic-like mechanism of domain formation in archaea and a role of intrinsic DNA structure in large-scale genome organization.
|
| Volume |
16(1)
|
| Pages |
1312
|
| Published |
2025-2-19
|
| DOI |
10.1038/s41467-025-56197-y
|
| PII |
10.1038/s41467-025-56197-y
|
| PMID |
39971902
|
| PMC |
PMC11840125
|
| MeSH |
Archaeal Proteins* / genetics
Archaeal Proteins* / metabolism
Chromosomes, Archaeal* / genetics
Chromosomes, Archaeal* / metabolism
DNA, Archaeal* / chemistry
DNA, Archaeal* / genetics
DNA, Archaeal* / metabolism
Genome, Archaeal
Nucleic Acid Conformation
Thermococcus* / genetics
Thermococcus* / metabolism
|
| Resource |
| General Microbes |
JCM9062 |
