| RRC ID |
89089
|
| Author |
Shi J, Zhao Z, Yang J, Cheng Z, Li H, Liu Y, Zhao G, Wu M, Du YL.
|
| Title |
Genome mining and characterization of a heme-dependent enzyme catalyzing intermolecular Nitrogen-Nitrogen bond formation in hydrazinosuccinic acid biosynthesis.
|
| Journal |
Synth Syst Biotechnol
|
| Abstract |
Nitrogen-nitrogen (N-N) bond-forming enzymes are rare but play vital roles in both primary and secondary metabolism. Guided by a nitric oxide synthase (NOS)-based genome mining strategy, we report the discovery and characterization of a new heme-dependent enzyme system that catalyzes intermolecular N-N bond formation. Using both in vivo and in vitro reconstitution approaches, we demonstrated that a protein complex, comprising a heme enzyme and a 2[4Fe-4S] ferredoxin partner, mediates the coupling of the α-amine group of l-aspartate with inorganic nitrogen oxide species, such as nitrite or nitric oxide, to generate hydrazinosuccinic acid, a key biosynthetic precursor in several natural product pathways. Structural modeling and site-directed mutagenesis suggest a plausible catalytic mechanism involving the formation of a reactive nitrogen intermediate, potentially a heme-bound nitrene species. These findings reveal a new family of N-N bond-forming biocatalysts that leverage inorganic nitrogen sources, offering valuable tools for genome mining and the synthetic biology.
|
| Volume |
10(4)
|
| Pages |
1421-1427
|
| Published |
2025-12-1
|
| DOI |
10.1016/j.synbio.2025.08.006
|
| PII |
S2405-805X(25)00117-6
|
| PMID |
40918557
|
| PMC |
PMC12408219
|
| Resource |
| General Microbes |
JCM35029 |
