| RRC ID |
89139
|
| Author |
Wang Y, Moriyama M, Koga R, Oguchi K, Hosokawa T, Takai H, Shigenobu S, Nikoh N, Fukatsu T.
|
| Title |
Tryptophanase disruption promotes insect-bacterium mutualism.
|
| Journal |
Nat Microbiol
|
| Abstract |
Animal-microorganism symbioses are omnipresent, with both partners often gaining benefits as mutualists. A single mutation in the carbon catabolite repression system in Escherichia coli enables mutualism with the stinkbug Plautia stali. Here we find that this mutation is not present in natural symbioses. Given that the carbon catabolite repression pathway affects the expression of >500 downstream genes, we investigated their role in mutualisms. We find that disruption of a single gene, tnaA, encoding tryptophanase makes E. coli mutualistic to P. stali, resulting in the accumulation of tryptophan and the reduction of toxic indole. A survey of wild populations of P. stali and other stinkbug species revealed that their typical microbial symbionts, Pantoea, consistently lack the tnaA gene. Some Pantoea species such as Pantoea ananatis retain the tnaA gene and cannot establish symbiosis with P. stali, but tnaA-disrupted P. ananatis partially restored the symbiotic capability. When a natural Pantoea mutualist of P. stali was transformed with a functional tna operon, its symbiotic capability reduced significantly. Our finding suggests that tryptophanase disruption may have facilitated the evolution of gut bacterial mutualists in insects.
|
| Volume |
11(3)
|
| Pages |
759-769
|
| Published |
2026-3-1
|
| DOI |
10.1038/s41564-026-02264-z
|
| PII |
10.1038/s41564-026-02264-z
|
| PMID |
41760926
|
| PMC |
PMC12962977
|
| MeSH |
Animals
Escherichia coli* / enzymology
Escherichia coli* / genetics
Escherichia coli* / physiology
Escherichia coli Proteins / genetics
Escherichia coli Proteins / metabolism
Heteroptera* / microbiology
Indoles / metabolism
Mutation
Operon
Pantoea* / enzymology
Pantoea* / genetics
Pantoea* / physiology
Symbiosis* / genetics
Tryptophan / metabolism
Tryptophanase* / genetics
Tryptophanase* / metabolism
|
| Resource |
| Prokaryotes E. coli |
|
