| RRC ID |
892
|
| Author |
Liu H, Kawabe A, Matsunaga S, Murakawa T, Mizukami A, Yanagisawa M, Nagamori E, Harashima S, Kobayashi A, Fukui K.
|
| Title |
Obtaining transgenic plants using the bio-active beads method.
|
| Journal |
J Plant Res
|
| Abstract |
Several methods of transformation are currently available for delivering exogenous DNA into animal and plant cells. In this study, a novel and efficient transformation system for DNA delivery/expression with a capacity to transport DNA of high molecular weight was developed. This system can overcome the shortcomings of traditional transformation methods such as Agrobacterium-mediated transformation, particle bombardment, and the electroporation method. The method developed in this study uses calcium alginate micro beads to immobilize DNA molecules in combination with polyethylene glycol treatment. In addition, it is simple and low-cost, and requires limited equipment. Using this method, we have successfully transformed tobacco plants, screening by kanamycin resistance. The transformed genes in the transformants were confirmed by PCR and Southern hybridization.
|
| Volume |
117(2)
|
| Pages |
95-9
|
| Published |
2004-4-1
|
| DOI |
10.1007/s10265-003-0141-3
|
| PMID |
15108034
|
| MeSH |
Alginates / pharmacology
Culture Media
Glucuronic Acid / pharmacology
Hexuronic Acids / pharmacology
Immobilization
Kanamycin / pharmacology
Kanamycin Resistance
Plant Proteins / genetics
Plants, Genetically Modified*
Plasmids
Polyethylene Glycols / pharmacology
Protoplasts
Tobacco / genetics*
Transformation, Genetic*
|
| IF |
2.185
|
| Times Cited |
10
|
|
WOS Category
|
PLANT SCIENCES
|
| Resource |
| Yeast |
|
