論文 - 詳細
| RRC ID | 89203 |
|---|---|
| 著者 | Komori R, Amano Y, Ogawa-Ohnishi M, Matsubayashi Y. |
| タイトル | Identification of tyrosylprotein sulfotransferase in Arabidopsis. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Tyrosine sulfation is a posttranslational modification common in peptides and proteins synthesized by the secretory pathway in most eukaryotes. In plants, this modification is critical for the biological activities of a subset of peptide hormones such as PSK and PSY1. In animals, tyrosine sulfation is catalyzed by Golgi-localized type II transmembrane proteins called tyrosylprotein sulfotransferases (TPSTs). However, no orthologs of animal TPST genes have been found in plants, suggesting that plants have evolved plant-specific TPSTs structurally distinct from their animal counterparts. To investigate the mechanisms of tyrosine sulfation in plants, we purified TPST activity from microsomal fractions of Arabidopsis MM2d cells, and identified a 62-kDa protein that specifically interacts with the sulfation motif of PSY1 precursor peptide. This protein is a 500-aa type I transmembrane protein that shows no sequence similarity to animal TPSTs. A recombinant version of this protein expressed in yeast catalyzed tyrosine sulfation of both PSY1 and PSK precursor polypeptide in vitro, indicating that the newly identified protein is indeed an Arabidopsis (At)TPST. AtTPST is expressed throughout the plant body, and the highest levels of expression are in the root apical meristem. A loss-of-function mutant of AtTPST displayed a marked dwarf phenotype accompanied by stunted roots, pale green leaves, reduction in higher order veins, early senescence, and a reduced number of flowers and siliques. Our results indicate that plants and animals independently acquired tyrosine sulfation enzymes through convergent evolution. |
| 巻・号 | 106(35) |
| ページ | 15067-72 |
| 公開日 | 2009-9-1 |
| DOI | 10.1073/pnas.0902801106 |
| PII | 0902801106 |
| PMID | 19666544 |
| PMC | PMC2736448 |
| MeSH | Amino Acid Sequence Arabidopsis / enzymology* Arabidopsis / growth & development Chromatography, Affinity Cloning, Molecular Conserved Sequence Gene Expression Regulation, Plant Humans Molecular Sequence Data Peptide Hormones / metabolism Protein Precursors / metabolism Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism Sequence Alignment Subcellular Fractions / metabolism Substrate Specificity Sulfotransferases / chemistry Sulfotransferases / genetics Sulfotransferases / isolation & purification Sulfotransferases / metabolism* |
| IF | 9.412 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | rpc00008 |