Reference - Detail
| RRC ID | 89231 |
|---|---|
| Author | Jamous M, Hosogane M, Huang X, Suzuki M, Hatano A, Shichino Y, Iwasaki S, Murayama K, Matsumoto M, Nakayama K. |
| Title | The ER anchoring and abundance of the EEF1B complex is affected by tissue-specific alternative EEF1D splicing. |
| Journal | Life Sci Alliance |
| Abstract |
The EEF1B complex plays a central role in translation elongation by reactivating EEF1A for the delivery of aminoacyl-tRNAs to the ribosome. Among its components, EEF1D undergoes alternative splicing to produce one long and several short isoforms, each with distinct N-terminal domains and tissue-specific expression patterns. Although the short isoforms are broadly expressed, their physiological functions remain poorly characterized. In this study, we show that short EEF1D isoforms containing exon 5 interact with the ER-resident scaffold protein KTN1 and RRBP1, thereby anchoring the EEF1B complex to the ER. Mass spectrometry analyses of FLAG-tagged EEF1D identified these interactions, and deletion of exon 5 disrupted ER anchoring, resulting in diffuse cytoplasmic localization of the EEF1B complex. In exon 5 KO mice, this altered localization was accompanied by a reduction in EEF1B subunit abundance in multiple tissues, including the liver, although global protein synthesis rates remained unchanged. Together, these findings uncover an ER-anchoring mechanism controlled by alternative splicing that shapes the spatial organization and abundance of the elongation machinery in vivo. |
| Volume | 9(8) |
| Published | 2026-8-1 |
| DOI | 10.26508/lsa.202503513 |
| PII | 9/8/e202503513 |
| PMID | 42230146 |
| PMC | PMC13231004 |
| MeSH | Alternative Splicing* / genetics Animals Endoplasmic Reticulum* / metabolism Exons / genetics Humans Mice Mice, Knockout Organ Specificity / genetics Peptide Elongation Factor 1* / genetics Peptide Elongation Factor 1* / metabolism Protein Isoforms / genetics Protein Isoforms / metabolism |
| IF | 2.622 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 17 |
| Altmetric score changes over past 6months | 5.8 |
| Resource | |
| Human and Animal Cells | Hepa 1-6(RCB1638) |