Reference - Detail
| RRC ID | 89249 |
|---|---|
| Author | Tsurumi Y, Masuda A, Xu J, Mon H, Kusakabe T, Lee JM. |
| Title | Structural and stability differences among GI norovirus virus-like particles produced in silkworm-baculovirus expression vector system. |
| Journal | Front Microbiol |
| Abstract |
Norovirus (NoV) is a leading cause of acute gastroenteritis worldwide, with genogroups I and II (GI and GII) most frequently detected. NoV Virus-like particles (VLPs) composed of the major capsid protein VP1 (~60 kDa) are essential for vaccine development, yet GI VLPs remain poorly characterized. In this study, VP1 from four epidemiologically relevant GI genotypes was expressed using the silkworm-baculovirus system, and purification conditions were optimized in a genotype-specific manner. Purified VLPs were analyzed using size-exclusion chromatography (SEC), dynamic light scattering (DLS), transmission electron microscopy (TEM), and differential scanning fluorimetry (DSF), and their functional epitopes confirmed via binding to histo-blood group antigens (HBGAs) mimics. Stability assessments revealed genotype-dependent differences in pH and thermal tolerance, including aggregation and structural transitions. Collectively, these findings define genotype-specific purification, structural characterization, and stability profiles for four GI NoV VLPs. This work provides the first systematic framework for understanding GI-specific constraints in VLP production and offers foundational data that directly inform the design and formulation of multivalent NoV vaccines. |
| Volume | 16 |
| Pages | 1739683 |
| Published | 2026-1-1 |
| DOI | 10.3389/fmicb.2025.1739683 |
| PMID | 41586362 |
| PMC | PMC12823906 |
| IF | 4.236 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Silkworms | d17 |