| RRC ID |
89254
|
| 著者 |
Iwanami N, Yoshida Y, Saito Y, Sakaguchi H, Yokoyama C, Matsuda M, Tachibana T.
|
| タイトル |
Distinct T cell lineages marked by expression of cd4 paralogues in medaka.
|
| ジャーナル |
Dev Comp Immunol
|
| Abstract |
T lymphocytes are classified into CD4+ and CD8+ T cells based on the glycoproteins expressed on their cell surface. Teleosts possess two CD4 paralogs, CD4-1 and CD4-2; however, their functional roles in T cell differentiation remain unclear. We generated fish mutant lines lacking the two cd4 paralog genes and genes encoding the two components of the CD8 co-receptor. The resulting phenotypes were analyzed using polyclonal antisera specific to medaka (Oryzias latipes) CD4-1 and CD8α, raised using a DNA immunization strategy. Our results indicate that cd4-2 deficiency does not affect the development of CD4-1+ T cells, providing genetic evidence for their distinct functions. Interestingly, in cd4-1 deficient mutants, the smaller compartment of CD4-1+ T cells was accompanied by a relative increase of CD8α+ T cells. Reciprocally, the lack or reduction of CD8α+ T cells caused by the deletion of cd8 genes was accompanied by a greater preponderance of CD4-1+ T cells. For the first time, our study provides direct genetic evidence for the interconnected differentiation programs of CD4+ and CD8+ T cells in teleost.
|
| ページ |
105646
|
| 公開日 |
2026-6-6
|
| DOI |
10.1016/j.dci.2026.105646
|
| PII |
S0145-305X(26)00102-3
|
| PMID |
42251920
|
| IF |
3.192
|
| リソース情報 |
| メダカ |
OK-cab (strain ID: MT830)
cab-Tg (lck:egfp), NK cell-specific lck promoter (strain ID: TG1582)
rag1 (del8) lacking mature B and T cells (strain ID: MT1583) |
