| RRC ID |
89340
|
| 著者 |
Yang G, Inoko M, Ogura K, Ishida-Ishihara S, Tsukada Y, Funahashi A, Sato M, Uehara R.
|
| タイトル |
Profiling cell proliferation after whole-genome duplication in human cells.
|
| ジャーナル |
Biol Open
|
| Abstract |
Though whole-genome duplication (WGD) contributes to cancer progression, the mechanism of post-WGD cell proliferation remains unclear. Here, using 6-day live-imaging, we analyzed the proliferation dynamics of more than 150 post-WGD HCT116 cell lineages. A quantitative comparison of mitotic patterns and cell fates between proliferative and non-proliferative lineages revealed that multipolar chromosome segregation in early mitosis is a potential key factor limiting the proliferative capacity of post-WGD progenies. Multipolar chromosome segregation correlated with suppressed post-WGD cell viability, particularly when accompanied by drastic chromosome loss or when it repeatedly occurred. Tracing proliferative lineages elucidated that they proliferated mainly by imposing the risk of multipolar chromosome segregation on one of two sub-lineages that formed after the first bipolar division. Meanwhile, a considerable proportion of proliferative lineages consisted entirely of progeny of early multipolar chromosome segregation events. Our results highlight key cellular events that determine the proliferation dynamics and diversity of post-WGD progenies, providing a fundamental reference for understanding WGD-associated biological processes.
|
| 巻・号 |
15(5)
|
| 公開日 |
2026-5-15
|
| DOI |
10.1242/bio.062568
|
| PII |
371660
|
| PMID |
42108858
|
| PMC |
PMC13267767
|
| MeSH |
Cell Lineage
Cell Proliferation* / genetics
Chromosome Segregation
Gene Duplication*
Genome, Human*
HCT116 Cells
Humans
Mitosis / genetics
|
| リソース情報 |
| ヒト・動物細胞 |
HCT116(RCB2979) |
