論文 - 詳細
| RRC ID | 89422 |
|---|---|
| 著者 | Oishi S, Doi S, Sekida T, Yamashita K, Yamada Y, Muramoto T. |
| タイトル | Genome editing across Dictyostelia species enables comparative functional genetics of social amoebas. |
| ジャーナル | Sci Rep |
| Abstract |
Gene manipulation is essential for understanding biological mechanisms, yet genetic modification in the social amoebas (Dictyostelia) has been largely limited to Dictyostelium discoideum. Here, we aimed to establish a CRISPR/Cas9-based genome-editing system applicable across the phylogenetic breadth of Dictyostelia, spanning Groups 1-4. Using an extrachromosomal CRISPR/Cas9 vector from D. discoideum, we disrupted stlA and pkaC in Polysphondylium violaceum and pkaC in two early-branching species, Heterostelium pallidum and Cavenderia fasciculata. In D. discoideum, co-introduction of donor oligos with the CRISPR vector enabled selection-free knockout generation of pkaC with 28.6% efficiency. In H. pallidum, where genome editing is typically inefficient, co-electroporation of donor oligos with the CRISPR/Cas9 vector followed by 4 days of drug selection increased the frequency of pkaC disruption from 0.9% to 8.3%. These results demonstrated that the D. discoideum CRISPR/Cas9 system can be extended across Dictyostelia, providing a versatile platform for comparative genetic and evolutionary developmental studies. |
| 巻・号 | 16(1) |
| 公開日 | 2026-2-5 |
| DOI | 10.1038/s41598-026-38605-5 |
| PII | 10.1038/s41598-026-38605-5 |
| PMID | 41644992 |
| PMC | PMC12929561 |
| MeSH | CRISPR-Cas Systems* Dictyostelium* / genetics Gene Editing* Genome, Protozoan* Phylogeny |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 21.5 |
| リソース情報 | |
| 細胞性粘菌 | S90889 S00222 S00492 |