論文 - 詳細
| RRC ID | 89470 |
|---|---|
| 著者 | Asaoka M, Hanyu-Nakamura K, Nakamura A, Kobayashi S. |
| タイトル | Maternal Nanos inhibits Importin-α2/Pendulin-dependent nuclear import to prevent somatic gene expression in the Drosophila germline. |
| ジャーナル | PLoS Genet |
| Abstract |
Repression of somatic gene expression in germline progenitors is one of the critical mechanisms involved in establishing the germ/soma dichotomy. In Drosophila, the maternal Nanos (Nos) and Polar granule component (Pgc) proteins are required for repression of somatic gene expression in the primordial germ cells, or pole cells. Pgc suppresses RNA polymerase II-dependent global transcription in pole cells, but it remains unclear how Nos represses somatic gene expression. Here, we show that Nos represses somatic gene expression by inhibiting translation of maternal importin-α2 (impα2) mRNA. Mis-expression of Impα2 caused aberrant nuclear import of a transcriptional activator, Ftz-F1, which in turn activated a somatic gene, fushi tarazu (ftz), in pole cells when Pgc-dependent transcriptional repression was impaired. Because ftz expression was not fully activated in pole cells in the absence of either Nos or Pgc, we propose that Nos-dependent repression of nuclear import of transcriptional activator(s) and Pgc-dependent suppression of global transcription act as a 'double-lock' mechanism to inhibit somatic gene expression in germline progenitors. |
| 巻・号 | 15(5) |
| ページ | e1008090 |
| 公開日 | 2019-5-1 |
| DOI | 10.1371/journal.pgen.1008090 |
| PII | PGENETICS-D-17-02179 |
| PMID | 31091233 |
| PMC | PMC6519790 |
| MeSH | Active Transport, Cell Nucleus Animals DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / cytology Drosophila melanogaster / genetics* Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism Female Fushi Tarazu Transcription Factors / genetics Fushi Tarazu Transcription Factors / metabolism Gene Expression Regulation, Developmental Male Nuclear Proteins / genetics* Nuclear Proteins / metabolism Ovary / cytology Ovary / growth & development Ovary / metabolism Ovum / cytology Ovum / metabolism* Positive Transcriptional Elongation Factor B / genetics Positive Transcriptional Elongation Factor B / metabolism RNA Polymerase II / genetics RNA Polymerase II / metabolism RNA-Binding Proteins / genetics* RNA-Binding Proteins / metabolism Spermatozoa / cytology Spermatozoa / metabolism* Testis / cytology Testis / growth & development Testis / metabolism Transcription Factors / genetics Transcription Factors / metabolism Transcription, Genetic alpha Karyopherins / genetics* alpha Karyopherins / metabolism |
| リソース情報 | |
| ショウジョウバエ | DGRC#119880 DGRC#119881 DGRC#119882 |