| RRC ID |
89473
|
| Author |
Matsuda K, Wang CH, Kakinuma H, Toyoda A, Shiraki T, Kawakami K, Kubo F.
|
| Title |
Molecular and functional dissection using CaMPARI-seq reveals the neuronal organization for dissociating optic flow-dependent behaviors.
|
| Journal |
Nat Commun
|
| Abstract |
Optic flow processing is critical for the visual control of body and eye movements in many animals. Rotational and translational binocular optic flow patterns need to be clearly distinguished to induce different behavior outputs. However, the specific neuron types and their connectivity involved in this computation remain unclear. Here, we developed a method to link the functional labeling using a photoconvertible calcium indicator called CaMPARI2 and single-cell RNA sequencing (CaMPARI-seq) to investigate the transcriptional profile of the pretectum, a center for processing optic flow in larval zebrafish. Using this technique, we identified a pretectal cluster expressing tcf7l2, which can be further classified into molecularly distinct subclusters. In vivo calcium imaging and cell ablation revealed that nkx1.2lb-positive pretectal neurons are commissural inhibitory neurons required for the optomotor response but not for the optokinetic response. Our genetic and functional dissection using CaMPARI-seq uncovered the neuronal organization essential for dissociating different optic flow-dependent behaviors.
|
| Volume |
17(1)
|
| Published |
2026-4-17
|
| DOI |
10.1038/s41467-026-71371-6
|
| PII |
10.1038/s41467-026-71371-6
|
| PMID |
41997902
|
| PMC |
PMC13090360
|
| MeSH |
Animals
Animals, Genetically Modified
Calcium / metabolism
Larva / physiology
Neurons* / metabolism
Neurons* / physiology
Optic Flow* / physiology
Single-Cell Analysis / methods
Single-Cell Gene Expression Analysis
Zebrafish* / genetics
Zebrafish* / physiology
Zebrafish Proteins / genetics
Zebrafish Proteins / metabolism
|
| Resource |
| Zebrafish |
gSA2AzGFF152B
UAS:GFP |
