論文 - 詳細
| RRC ID | 89484 |
|---|---|
| 著者 | Imamura T, Shigehisa R, Miyazato A, Matsumura N, Miyaki K, Segawa T, Yoshizumi M, Takagi H, Yamaguchi T, Ohki S, Mori M. |
| タイトル | A cyclo-DOPA 6-O-glucosyltransferase-mediated route for gomphrenin I biosynthesis in Basella alba and Gomphrena globosa. |
| ジャーナル | New Phytol |
| Abstract |
Betacyanins are red pigments characteristic of Caryophyllales and show considerable structural diversity, yet the enzymatic basis underlying 6-O-glucosylated betacyanins, such as gomphrenin I, has remained unclear. In particular, how alternative glucosylation patterns contribute to betacyanin diversification is poorly understood. Here, we identified cyclo-DOPA glucosyltransferases from Basella alba and Gomphrena globosa and examined their roles in gomphrenin I biosynthesis using transient expression assays and tobacco BY-2 cell systems. Phylogenetic analyses, structural modelling, and site-directed mutagenesis were employed to investigate their functional and structural characteristics. BacDOPA5/6GTs catalysed both 5-O- and 6-O-glucosylation of cyclo-DOPA, leading to the production of betanin and gomphrenin I, whereas GgcDOPA6GT specifically mediated gomphrenin I formation. These enzymes belong to distinct subclades within the cDOPA-GT family, and mutational analyses demonstrated essential roles for conserved histidine residues and an α-helical region adjacent to the catalytic site. Thermal stability analyses further showed that gomphrenin I is more thermally stable than betanin, likely due to the formation of an intramolecular hydrogen bond. Together, these results reveal an additional cDOPA6GT-mediated route for gomphrenin I biosynthesis and provide insight into the diversification and functional specialisation of betacyanins, linking the position of glucosylation to pigment stability and biochemical properties. |
| 公開日 | 2026-6-7 |
| DOI | 10.1111/nph.71340 |
| PMID | 42252617 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 21 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 7.3 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | rpc00001 |