論文 - 詳細
| RRC ID | 89521 |
|---|---|
| 著者 | Kamarulzaman L, Kim S, Hidaka T, Tsuchida M, Taniguchi Y. |
| タイトル | Molecular profiling of the single-cell proteome via gel electrophoresis and 3D single-molecule imaging. |
| ジャーナル | Nat Commun |
| Abstract |
Recent advances in shotgun proteomics and immunoassays have yielded powerful single-cell proteomics technologies. However, current methods lack the sensitivity required to comprehensively quantify protein abundances in individual cells. Here, we present single-cell PAGE-PISA, an ultra-sensitive proteome profiling strategy that combines gel electrophoresis with 3D single-molecule fluorescence imaging. Our approach labels all proteins in single cells with fluorescent dyes, separates them by electrophoresis, and counts with single-molecule resolution. This technique quantified over 107 protein copies from a single mammalian cell with the sensitivity to detect low-abundance proteins down to 105 copies per species. Single-cell PAGE-PISA successfully classified cells into distinct cell types based on their proteomic profiles. Furthermore, our single-cell proteome data strongly correlated with predicted developmental states during cardiomyocyte differentiation, providing complementary information to single-cell transcriptome data. Together, single-cell PAGE-PISA enables highly sensitive and quantitative proteome profiling at the single-cell level, capturing subtle proteomic differences that distinguish diverse cellular states. |
| 巻・号 | 17(1) |
| 公開日 | 2026-7-8 |
| DOI | 10.1038/s41467-026-74840-0 |
| PII | 10.1038/s41467-026-74840-0 |
| PMID | 42420286 |
| PMC | PMC13346896 |
| MeSH | Animals Cell Differentiation Electrophoresis, Polyacrylamide Gel* / methods Fluorescent Dyes Myocytes, Cardiac / cytology Myocytes, Cardiac / metabolism Proteome* / metabolism Proteomics* / methods Single Molecule Imaging* / methods Single-Cell Analysis* / methods |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 30 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 19.1 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) PC-3(RCB2145) |