Reference - Detail
| RRC ID | 89566 |
|---|---|
| Author | Fuse M. |
| Title | Development of fish scale collagen-immobilized poly(lactic acid) films using chemical treatment with hexamethylenediamine. |
| Journal | J Oral Sci |
| Abstract |
PURPOSE:This study aimed to evaluate how immobilizing tilapia fish scale type 1 collagen onto poly(lactic acid) (PLA), following chemical modification with hexamethylenediamine (HMDA), affects the adhesion and proliferation of mouse osteoblast-like cells (OLCs). METHODS:PLA films were chemically modified by HMDA immersion to introduce amino groups (-NH2) (PLA-NH2). Tilapia fish scale type 1 collagen was subsequently immobilized onto the PLA-NH2 surface via a water-soluble carbodiimide condensation reaction, producing (TFColl-PLA-NH2). Surface properties of the films were characterized using contact angle measurements and X-ray photoelectron spectroscopy (XPS). Biological evaluation was conducted by culturing OLCs on PLA, PLA-NH2, and TFColl-PLA-NH2 films, followed by quantification of cell numbers. RESULTS:The contact angles of PLA-NH2 and TFColl-PLA-NH2 were significantly lower than PLA (P < 0.05). XPS confirmed successful immobilization of tilapia collagen onto the PLA surface. At all time points, TFColl-PLA-NH2 showed the highest cell numbers, followed by PLA-NH2, while PLA had the lowest (P < 0.05). PLA-NH2 promoted calcification more effectively than PLA (P < 0.05), and TFColl- PLA-NH2 further enhanced calcification compared with PLA-NH2 (P < 0.05). CONCLUSION:PLA surface treatment with HMDA is an effective and straightforward method. Tilapia fish scale type 1 collagen has potential for application as a novel biomaterial source. |
| Volume | 68(3) |
| Pages | 152-157 |
| Published | 2026-1-1 |
| DOI | 10.2334/josnusd.26-0015 |
| PMID | 42457555 |
| MeSH | Animal Scales* / chemistry Animals Cell Adhesion Cell Proliferation Collagen* / chemistry Diamines* / chemistry Lactic Acid* / chemistry Mice Osteoblasts / cytology Photoelectron Spectroscopy Polyesters* / chemistry Polymers* / chemistry Surface Properties Tilapia |
| Resource | |
| Human and Animal Cells | MC3T3-E1(RCB1126) |