| RRC ID |
89614
|
| 著者 |
Wakigawa T, Mito M, Fang Q, Itoh Y, Shinkai Y, Iwasaki S.
|
| タイトル |
Mitochondrial translation termination, recycling, reinitiation, and rescue for in-frame and out-of-frame contexts.
|
| ジャーナル |
Nat Commun
|
| Abstract |
Because mitochondria diverged from a bacterial ancestor during evolution, the mitochondrial protein synthesis system includes both mRNAs and translation factors with unique characteristics. However, the molecular mechanisms underlying translation termination, recycling, and quality control remain unclear. Here, via high-resolution mitochondrial Ribo-Seq and Disome-Seq, we reveal: the specificity of release factors for different kinds of stop codons; the role of mtRF1 in vertebrates, which do not have noncanonical stop codons in their main translons; the recycling-coupled translation of internal translons; and the rescue of mitoribosomes in the early elongation stage. mtRF1L recognizes all stop codons, whereas mtRF1 recognizes only AGA/AGG noncanonical stop codons. Additionally, mtRF1 terminates the translation of out-of-frame translons that end with AGA/AGG. We also found that mtRRF and mtIF3 are required for mitoribosome recycling on stop codons and for the reinitiation of internal translon translation. Mitoribosomes that stall at the start codons and/or at the early elongation phase are major substrates of the rescue factors ICT1, mtRF-R, and mtRES1. Moreover, HEMK1-mediated methylation of release factors enhances the termination reaction on stop codons. Our results provide insights into the mitoribosome dynamics that are associated with the completion of protein synthesis.
|
| 巻・号 |
17(1)
|
| 公開日 |
2026-7-21
|
| DOI |
10.1038/s41467-026-75248-6
|
| PII |
10.1038/s41467-026-75248-6
|
| PMID |
42481489
|
| MeSH |
Animals
Codon, Terminator / genetics
Codon, Terminator / metabolism
Humans
Mitochondria* / genetics
Mitochondria* / metabolism
Mitochondrial Proteins* / genetics
Mitochondrial Proteins* / metabolism
Mitochondrial Ribosomes* / metabolism
Peptide Chain Initiation, Translational*
Peptide Chain Termination, Translational*
Peptide Termination Factors / genetics
Peptide Termination Factors / metabolism
Protein Biosynthesis*
RNA, Messenger / genetics
RNA, Messenger / metabolism
|
| リソース情報 |
| ヒト・動物細胞 |
HeLa |
