| RRC ID |
89645
|
| Author |
Kozono Y, Mikami K, Yorimoto S, Hayashi T, Okamura H, Qian Q, Hoshino R, Kamiyama T, Sanaki Y, Asaoka M, Ohsawa S, Azuma S, Iwasaki YW, Hayashi M, Hayashi Y, Shigenobu S, Niwa R, Kobayashi S.
|
| Title |
EC-isHCR: A rapid method for in situ hybridization chain reaction in diverse animal samples.
|
| Journal |
Methods
|
| Abstract |
The in situ hybridization chain reaction (isHCR) visualizes RNA across multiple spatial scales, from organs to subcellular structures, in diverse samples. We previously proposed a rapid protocol, EC-isHCR, for Drosophila embryos and ovaries. Whether EC-isHCR retains the features of conventional isHCR, including wide-spatial-scale analyses in various samples, however, has remained unclear. Here, we show that EC-isHCR enables robust RNA detection in a broad range of samples, such as whole-mount fruit fly, parasitoid wasp, and aphid preparations; paraffin sections of trout; frozen mouse sections; and human cultured cells. Moreover, EC-isHCR enabled detection of subcellular RNA localization. EC-isHCR also visualized association of RNA with phase-separated condensates in fruit fly embryos and detected the protrusion-enriched mRNA in HeLa cells. To broaden the applicability of EC-isHCR, we developed an automated probe design tool (https://github.com/ShuntaYorimoto/hcrkit). By combining this tool with EC-isHCR, we provide a fast and versatile framework to visualize mRNAs. This framework will help reduce the barrier to using fast isHCR and thereby facilitate research across diverse areas of the life sciences.
|
| Volume |
254
|
| Pages |
59-69
|
| Published |
2026-6-29
|
| DOI |
10.1016/j.ymeth.2026.06.007
|
| PII |
S1046-2023(26)00154-4
|
| PMID |
42372957
|
| Resource |
| Human and Animal Cells |
HeLa(RCB0007) |
