| RRC ID |
89662
|
| 著者 |
Besson D, Vaur S, Vazquez S, Tournier S, Gachet Y, Birot A, Claverol S, Marston AL, Damdimopoulos A, Ekwall K, Javerzat JP.
|
| タイトル |
Interplay between cohesin and TORC1 links chromosome segregation and gene expression to environmental changes.
|
| ジャーナル |
Elife
|
| Abstract |
Cohesin is a DNA tethering complex essential for chromosome structure and function. In fission yeast, defects in the cohesin loader Mis4 result in chromosome segregation defects and dysregulated expression of genes near chromosome ends. A genetic screen for suppressors of the thermosensitive growth defect of mis4-G1487D identified several hypomorphic mutants of the Target of Rapamycin Complex 1 (TORC1), a conserved kinase that integrates cellular signals to regulate growth and metabolism through substrate-specific phosphorylation. Here, we demonstrate that the TORC1 pathway modulates cohesin functions in chromosome segregation and gene expression. In the context of compromised cohesin loading, the incidence of chromosome segregation defects was modulated by the growth medium in a TORC1-dependent manner. Pharmacological or genetic downregulation of TORC1 activity restored cohesin binding to its chromosomal sites and improved mitotic chromosome segregation. Notably, reduced TORC1 activity also increased cohesin binding and chromosome transmission fidelity in wild-type cells. These results suggest that environmental cues influence chromosome stability via TORC1. Biochemically, TORC1 co-purified with cohesin and reduced TORC1 activity correlated with decreased phosphorylation of specific residues on Mis4 and cohesin. Mutations in cohesin that mimic the non-phosphorylated state mirrored the effects of TORC1 downregulation, showing that TORC1 is part of the network that controls cohesin phosphorylation to modulate its functions. Finally, we show that the functional interaction between TORC1 and Mis4 extends to the regulation of stress-responsive genes. Our findings reveal a TORC1-cohesin link that may facilitate cellular adaptation to environmental changes. Given that TORC1 inhibitors and calorie restriction extend lifespan in diverse species, this connection raises the intriguing possibility that cohesin-mediated changes in chromosome structure contribute to these effects.
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| 巻・号 |
14
|
| 公開日 |
2026-6-1
|
| DOI |
10.7554/eLife.108275
|
| PII |
108275
|
| PMID |
42223017
|
| PMC |
PMC13225845
|
| MeSH |
Cell Cycle Proteins* / metabolism
Chromosomal Proteins, Non-Histone* / metabolism
Chromosome Segregation*
Cohesins
Gene Expression Regulation, Fungal*
Mechanistic Target of Rapamycin Complex 1* / metabolism
Phosphorylation
Schizosaccharomyces* / genetics
Schizosaccharomyces* / metabolism
Schizosaccharomyces* / physiology
Schizosaccharomyces pombe Proteins* / genetics
Schizosaccharomyces pombe Proteins* / metabolism
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| リソース情報 |
| 酵母 |
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