| RRC ID |
89774
|
| Author |
Hatano H, Sawada M, Nakamura N, Kitsukawa M, Hayakawa A, Kondo H, Kawashima I.
|
| Title |
Terminal sterilization of food-grade coatings for cellular agriculture: robustness against water quality and regulatory feasibility.
|
| Journal |
Biotechnol Rep (Amst)
|
| Abstract |
High production costs remain a major obstacle to the commercialization of cell-based foods, primarily due to the reliance on costly pharmaceutical-grade reagents and infrastructure. This study introduces a practical solution using a dual technical/regulatory approach. We developed iCoater, a food-grade coating agent manufactured via terminal heat sterilization, a standard in food processing. This process results in no detectable bacterial growth and 12-month stability without filtration. In performance tests using primary duck liver-derived cells, iCoater showed efficacy in cell attachment and proliferation. Furthermore, we confirmed that this performance and low endotoxin levels were maintained when the tested water sources covered a wide hardness range from soft (< 1.0 mg/L) to hard (1468 mg/L) water. By aligning the food-grade manufacturing process, we proposed the regulatory pathway under Japanese legislation, identifying "gelatin-processed food" as a realistic and transparent labeling strategy. This integrated approach provides a practical reference for future developments in cellular agriculture.
|
| Volume |
51
|
| Pages |
e00976
|
| Published |
2026-9-1
|
| DOI |
10.1016/j.btre.2026.e00976
|
| PII |
S2215-017X(26)00031-7
|
| PMID |
42620750
|
| PMC |
PMC13486997
|
| Resource |
| Human and Animal Cells |
293T(RCB2202)
C2C12(RCB0987) |
