RRC ID 89777
Author Obana S, Minato J, Takahashi S, Itakura S, Nishikawa M, Kusamori K.
Title Human iPSC-derived mesenchymal progenitor cell sheets promote tissue formation after xenotransplantation.
Journal iScience
Abstract Mesenchymal stromal cell (MSC) sheets are a promising platform for regenerative therapy, but their in vivo survival and capacity to form tissue-like structures remain poorly understood. Here, we investigated whether sheets derived from human induced pluripotent stem cell (iPSC)-derived mesenchymal progenitor cells (hiPS-MPCs) can survive and form tissue-like structures after xenotransplantation. hiPS-MPCs were differentiated from human iPSCs, validated by surface marker expression and multilineage differentiation potential, and assembled into intact cell sheets. Mouse MSC, fibroblast, and hiPS-MPC sheets were transplanted subcutaneously into immunocompetent mice. By day 7, mouse MSC and hiPS-MPC sheets formed organized vascularized tissue-like structures, whereas fibroblast sheets frequently induced inflammatory responses and showed limited persistence. Notably, hiPS-MPC-derived tissues exhibited reduced immune cell infiltration and higher expression of anti-inflammatory cytokines than fibroblast tissues. These findings demonstrate that hiPS-MPC sheets generate vascularized tissues in vivo and support survival across species, highlighting their potential as a scalable regenerative therapy platform.
Volume 29(8)
Pages 117128
Published 2026-8-21
DOI 10.1016/j.isci.2026.117128
PII S2589-0042(26)02506-X
PMID 42620823
PMC PMC13486853
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Total number of mentions 2
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Resource
Human and Animal Cells NIH3T3-3-4(RCB1862)