論文 - 詳細
| RRC ID | 89847 |
|---|---|
| 著者 | Poirier W, Faway É, Yamada T, Ozawa K, Maréchal F, Monod M, Poumay Y, Mignon B. |
| タイトル | Subtilisin 6 From the Dermatophyte Trichophyton benhamiae Is a Marker of Infection but Not a Unique Virulence Factor. |
| ジャーナル | Mycoses |
| Abstract |
BACKGROUND:Trichophyton benhamiae is a common dermatophyte whose natural host is the guinea pig and which causes highly inflammatory skin lesions in humans. The subtilisin 6 (SUB6) of this fungus belongs to a family of 12 SUB genes. Its encoding gene, overexpressed in vivo but not in vitro, has been considered a potentially important virulence factor, but its role in pathogenesis remains to be elucidated. OBJECTIVES:The aim of this study was to assess the role of T. benhamiae SUB6 in virulence in a mouse skin infection model. METHODS:To assess the contribution of SUB6 to virulence, SUB6-deleted (ΔSUB6) and complemented strains were generated by genetic transformation. The pathogenicity of these strains was compared with that of the parental strain in vivo in mice, based on the evolution of skin symptoms, histopathological lesions and molecular analyses targeting the expression of host pro-inflammatory genes and fungal genes encoding subtilisins from the same family as SUB6. RESULTS:The ΔSUB6 strain induced superficial skin signs and histopathological inflammatory lesions similar to those caused by the parental strain. Significant overexpression of the SUB1, SUB3, SUB8 and SUB10 genes in the tissues was observed regardless of the strain tested, with no difference between these strains, reflecting the absence of any compensatory mechanism among subtilisins. CONCLUSIONS:SUB6 appears to be more of a marker of fungal infection than a virulence factor, at least acting alone. |
| 巻・号 | 68(2) |
| ページ | e70037 |
| 公開日 | 2025-2-1 |
| DOI | 10.1111/myc.70037 |
| PMID | 39980131 |
| MeSH | Animals Biomarkers Disease Models, Animal Female Fungal Proteins* / genetics Fungal Proteins* / metabolism Mice Mice, Inbred BALB C Skin / microbiology Skin / pathology Subtilisin* / genetics Subtilisin* / metabolism Subtilisins* / genetics Subtilisins* / metabolism Tinea* / microbiology Tinea* / pathology Trichophyton* / enzymology Trichophyton* / genetics Trichophyton* / pathogenicity Virulence Virulence Factors* / genetics Virulence Factors* / metabolism |
| リソース情報 | |
| 病原真核微生物 | Trichophyton benhamiae IFM 66349 |