| RRC ID |
89877
|
| Author |
Baltaci OF, Usseglio Gaudi A, Dudczig S, Wang W, Paterson S, Rondon-Galeano MC, Lim YW, Rae J, Lagendijk A, Parton RG, Farley A, Hogan BM.
|
| Title |
Mural cells protect the adult brain from hemorrhage but do not control the blood-brain barrier in developing zebrafish.
|
| Journal |
Elife
|
| Abstract |
The blood-brain barrier (BBB) protects the brain from circulating metabolites and plays central roles in neurological diseases. Endothelial cells (ECs) of the BBB are enwrapped by mural cells including pericytes and vascular smooth muscle cells (vSMCs) that regulate angiogenesis, vessel stability and barrier function. To explore mural cell control of the BBB, we investigated neurovascular phenotypes in zebrafish pdgfrb mutants that lack brain pericytes and vSMCs. As expected, mutants showed an altered cerebrovascular network with mispatterned capillaries. Unexpectedly, mutants displayed no BBB leakage at larval stages of development. This suggests that pericytes and vSMCs are not essential for normal BBB function in developing zebrafish. Instead, we observed juvenile and adult BBB disruption occurring at 'hotspot' focal hemorrhages at large vessel aneurysms. ECs at leakage hotspots showed induction of caveolae on abluminal surfaces and structural defects including basement membrane thickening and disruption. Our work suggests that capillary pericytes primarily regulate cerebrovascular patterning in development and vSMCs of major arteries protect from hemorrhage and BBB breakdown in older zebrafish. The fact that young zebrafish have a functional BBB in the absence of mural cells calls for renewed interrogation of mural cell control of the BBB throughout vertebrate evolution.
|
| Volume |
14
|
| Published |
2026-6-1
|
| DOI |
10.7554/eLife.104061
|
| PII |
104061
|
| PMID |
42223470
|
| PMC |
PMC13225842
|
| MeSH |
Animals
Blood-Brain Barrier*
Brain*
Endothelial Cells* / physiology
Muscle, Smooth, Vascular*
Myocytes, Smooth Muscle* / physiology
Pericytes* / metabolism
Pericytes* / physiology
Receptor, Platelet-Derived Growth Factor beta / genetics
Receptor, Platelet-Derived Growth Factor beta / metabolism
Zebrafish* / growth & development
|
| Resource |
| Zebrafish |
UAS:RFP |
