| RRC ID |
96
|
| 著者 |
Kanatsu-Shinohara M, Toyokuni S, Shinohara T.
|
| タイトル |
CD9 is a surface marker on mouse and rat male germline stem cells.
|
| ジャーナル |
Biol Reprod
|
| Abstract |
Spermatogenesis is dependent on a small population of stem cells. Despite the biological significance of spermatogonial stem cells, their analysis has been hampered by their scarcity. However, spermatogonial stem cells can be enriched by selection with an antibody against cell-surface molecules. In this investigation, we searched for new antigens expressed on spermatogonial stem cells. Using the spermatogonial transplantation technique, we examined expression of the CD9 molecule, which is commonly expressed on stem cells of other tissues. Selection of both mouse and rat testis cells with anti-CD9 antibody resulted in 5- to 7-fold enrichment of spermatogonial stem cells from intact testis cells, indicating that CD9 is commonly expressed on spermatogonial stem cells of both species. Therefore, CD9 may be involved in the common machinery in stem cells of many self-renewing tissues, and the identification of a common surface antigen on spermatogonial stem cells of different species has important implications for the development of a technique to enrich stem cells from other mammalian species.
|
| 巻・号 |
70(1)
|
| ページ |
70-5
|
| 公開日 |
2004-1-1
|
| DOI |
10.1095/biolreprod.103.020867
|
| PII |
biolreprod.103.020867
|
| PMID |
12954725
|
| MeSH |
Animals
Antibodies
Antigens, CD / analysis
Antigens, CD / immunology
Antigens, CD / metabolism*
Biomarkers
Immunomagnetic Separation*
Male
Membrane Glycoproteins / analysis
Membrane Glycoproteins / immunology
Membrane Glycoproteins / metabolism*
Mice
Mice, Nude
Mice, Transgenic
Rats
Rats, Inbred Strains
Spermatogenesis / physiology
Spermatogonia / cytology*
Stem Cells / chemistry
Stem Cells / cytology*
Stem Cells / metabolism*
Tetraspanin 29
|
| IF |
3.322
|
| 引用数 |
187
|
|
WOS 分野
|
REPRODUCTIVE BIOLOGY
|
| リソース情報 |
| ラット |
SD-Tg(CAG-EGFP)4Osb(strainID=559) |
