RRC ID 98
Author Kuwada M, Kawashima R, Furudate S, Sugano S, Maki J.
Title One-step analysis of testis steroidogenesis from neonatal exposure to synthetic estrogen by normal-phase HPLC.
Journal Biomed. Chromatogr.
Abstract Neonatal exposure to synthetic estrogen endocrine disruptors or estrogen-receptor inhibitors induces developmental abnormalities in the male reproductive system. To investigate whether neonatal exposure affects spermatogenesis in juvenile and pubertal testis, Sprague-Dawley rat pups were given synthetic estrogen endocrine disruptors or estrogen-receptor inhibitors by a single injection on the day of birth at concentrations ranging between 2 to 40 mm, and sacrificed on day 21 (juvenile), 35 (prepuberty) or 50 (puberty). The testes were weighed and examined histologically at each stage. Further, the metabolites of steroidogenesis were analyzed using normal-phase high performance liquid chromatography. Neonatal exposure significantly reduced testis weights and steroidogenesis to one- fifth to one-half of that of the juvenile control, and further suppressed irreversible steroidogenesis and spermatogenesis during puberty.
Volume 18(4)
Pages 234-9
Published 2004-5
DOI 10.1002/bmc.309
PMID 15162385
MeSH Animals Animals, Newborn Chromatography, High Pressure Liquid / methods* Diethylstilbestrol / pharmacology Endocrine Glands / drug effects Estrogens / pharmacology* Female Male Organ Size / drug effects* Rats Rats, Sprague-Dawley Receptors, Estrogen / antagonists & inhibitors Receptors, Estrogen / metabolism Sesame Oil Sexual Maturation Spermatogenesis / drug effects Steroids / analysis Steroids / biosynthesis* Testis / drug effects* Testis / metabolism Testis / pathology
IF 1.688
Times Cited 2
WOS Category CHEMISTRY, ANALYTICAL PHARMACOLOGY & PHARMACY BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Rats