| RRC ID |
98
|
| 著者 |
Kuwada M, Kawashima R, Furudate S, Sugano S, Maki J.
|
| タイトル |
One-step analysis of testis steroidogenesis from neonatal exposure to synthetic estrogen by normal-phase HPLC.
|
| ジャーナル |
Biomed Chromatogr
|
| Abstract |
Neonatal exposure to synthetic estrogen endocrine disruptors or estrogen-receptor inhibitors induces developmental abnormalities in the male reproductive system. To investigate whether neonatal exposure affects spermatogenesis in juvenile and pubertal testis, Sprague-Dawley rat pups were given synthetic estrogen endocrine disruptors or estrogen-receptor inhibitors by a single injection on the day of birth at concentrations ranging between 2 to 40 mm, and sacrificed on day 21 (juvenile), 35 (prepuberty) or 50 (puberty). The testes were weighed and examined histologically at each stage. Further, the metabolites of steroidogenesis were analyzed using normal-phase high performance liquid chromatography. Neonatal exposure significantly reduced testis weights and steroidogenesis to one- fifth to one-half of that of the juvenile control, and further suppressed irreversible steroidogenesis and spermatogenesis during puberty.
|
| 巻・号 |
18(4)
|
| ページ |
234-9
|
| 公開日 |
2004-5-1
|
| DOI |
10.1002/bmc.309
|
| PMID |
15162385
|
| MeSH |
Animals
Animals, Newborn
Chromatography, High Pressure Liquid / methods*
Diethylstilbestrol / pharmacology
Endocrine Glands / drug effects
Estrogens / pharmacology*
Female
Male
Organ Size / drug effects*
Rats
Rats, Sprague-Dawley
Receptors, Estrogen / antagonists & inhibitors
Receptors, Estrogen / metabolism
Sesame Oil
Sexual Maturation
Spermatogenesis / drug effects
Steroids / analysis
Steroids / biosynthesis*
Testis / drug effects*
Testis / metabolism
Testis / pathology
|
| IF |
1.728
|
| 引用数 |
2
|
|
WOS 分野
|
CHEMISTRY, ANALYTICAL
PHARMACOLOGY & PHARMACY
BIOCHEMICAL RESEARCH METHODS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ラット |
|
