RRC ID 997
著者 Sharmin S, Ohori A, Sano A, Kamei K, Yamaguchi M, Takeo K, Uno J, Nishimura K, Miyaji M.
タイトル Histoplasma capsulatum variety duboisii isolated in Japan from an HIV-infected Ugandan patient.
ジャーナル Nihon Ishinkin Gakkai Zasshi
Abstract A strain of Histoplasma capsulatum var. duboisii (deposited as IFM 50954 in Chiba University) was isolated from the cerebrospinal fluid of a female Ugandan patient infected with HIV. The isolate had in vitro urease activity on Christensen's urea agar slants, although the common belief is that H. capsulatum var. duboisii is urease negative, and is, considered one of the characteristic markers that distinguishes the three varieties of H. capsulatum. Forty H. capsulatum var. capsulatum, five H. capsulatum var. duboisii, and five H. capsulatum var. farciminosum isolates were evaluated for urease activity on Christensen's urea agar slants and for other qualitative and quantitative urease assays of activity. All 50 isolates of H. capsulatum used in this study were positive for urease activity, suggesting that urease activity may be universal characteristic of H. capsulatum. We also compared the urease activity and pathogenicity of seven H. capsulatum isolates that convert into yeast-form cells. Although isolate IFM 50954 showed moderate virulence in mice and moderate urease activity among tested H. capsulatum isolates, there was no correlation between level of urease activity and pathogenicity. In addition, scanning electron microscopy revealed that some microconidia of isolate IFM 50954 formed "double-cell" configurations that were attached to each other by narrow bases.
巻・号 44(4)
ページ 299-306
公開日 2003-1-1
DOI 10.3314/jjmm.44.299
PMID 14615797
MeSH Animals Female HIV Infections / microbiology* Histoplasma / isolation & purification* Histoplasma / pathogenicity Humans Japan Male Mice Mice, Inbred Strains Uganda / ethnology Urease / analysis
リソース情報
病原微生物 IFM 5396 IFM 5397 IFM 5398 IFM 5399 IFM 5400 IFM 5401 IFM 5402 IFM 5403 IFM 5404 IFM 5405 IFM 5406 IFM 5407 IFM 5408 IFM 5410 IFM 5411 IFM 5412 IFM 5413 IFM 5414 IFM 5415 IFM 5416 IFM 5417 IFM 5418 IFM 5844 IFM 40009 IFM 40752 IFM 41323 IFM 41329 IFM 41330 IFM 41331 IFM 41332 IFM 41333 IFM 41334 IFM 41335 IFM 41612 IFM 41618 IFM 41619 IFM 46003 IFM 46004 IFM 46159 IFM 47750 IFM 49109 IFM 49110 IFM 49200 IFM 49721 IFM 50248 IFM 50249 IFM 50250 IFM 50251 IFM 50954 IFM 50955 IFM 50958 IFM 50959