| RRC ID |
999
|
| 著者 |
Biswas SK, Wang L, Yokoyama K, Nishimura K.
|
| タイトル |
Molecular analysis of Cryptococcus neoformans mitochondrial cytochrome b gene sequences.
|
| ジャーナル |
J Clin Microbiol
|
| Abstract |
Mitochondrial cytochrome b genes (cyt b) of 40 strains of Cryptococcus neoformans were partially sequenced to determine the genetic relations. With the exception of the type strain of C. neoformans var. neoformans, all strains contained introns in their sequences. Analysis of 386 bp of coding sequence from each strain under investigation revealed a total of 27 (6.99%) variable nucleotide sites and categorized isolates of C. neoformans into nine cyt b types. C. neoformans var. gattii included cyt b types I to V, and C. neoformans var. neoformans comprised types VI to IX. cyt b types were correlated with serotypes. All strains with cyt b types I, IV, and V were serotype B. All other strains except IFM 5878 (serotype B) with cyt b types II and III were serotype C. Serotype D strains had cyt b types VI and IX, and serotype A strains were cyt b type VIII. Of four serotype AD strains, one was cyt b type VII and the remaining three were type VIII. The phylogenetic tree based on deduced amino acid sequences divided the strains only into C. neoformans var. neoformans and C. neoformans var. gattii. These results indicate that cyt b sequences are effective for DNA typing as well as phylogenetic analysis of C. neoformans.
|
| 巻・号 |
41(12)
|
| ページ |
5572-6
|
| 公開日 |
2003-12-1
|
| DOI |
10.1128/JCM.41.12.5572-5576.2003
|
| PMID |
14662942
|
| PMC |
PMC308988
|
| MeSH |
Base Sequence
Cryptococcus neoformans / classification
Cryptococcus neoformans / genetics*
Cryptococcus neoformans / isolation & purification
Cytochromes b / genetics*
DNA Primers
Genes, Fungal
Humans
Mitochondria / enzymology*
Mitochondria / genetics
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction / methods
Sequence Alignment
Sequence Homology, Nucleic Acid
Serotyping / methods
|
| IF |
5.897
|
| 引用数 |
16
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 病原微生物 |
IFM 5505
IFM 5506
IFM 5814
IFM 5815
IFM 5844
IFM 5845
IFM 5854
IFM 5855
IFM 5856
IFM 5857
IFM 5873
IFM 5875
IFM 5878
IFM 5881
IFM 5889
IFM 45708
IFM 45737
IFM 45756
IFM 46082
IFM 46090
IFM 46132
IFM 46554
IFM 46572
IFM 46729
IFM 46734
IFM 47258
IFM 48634
IFM 48635
IFM 48636
IFM 48637
IFM 48638
IFM 48639
IFM 48640
IFM 48641
IFM 48642
IFM 48643
IFM 48818
IFM 48819
IFM 48820
IFM 48821
IFM 48822 |
