RRC ID |
21129
|
著者 |
Verbitskiy D, Merwe JA, Zehrmann A, Härtel B, Takenaka M.
|
タイトル |
The E-class PPR protein MEF3 of Arabidopsis thaliana can also function in mitochondrial RNA editing with an additional DYW domain.
|
ジャーナル |
Plant Cell Physiol
|
Abstract |
In plants, RNA editing is observed in mitochondria and plastids, changing selected C nucleotides into Us in both organelles. We here identify the PPR (pentatricopeptide repeat) protein MEF3 (mitochondrial editing factor 3) of the E domain PPR subclass by genetic mapping of a variation between ecotypes Columbia (Col) and Landsberg erecta (Ler) in Arabidopsis thaliana to be required for a specific RNA editing event in mitochondria. The Ler variant of MEF3 differs from Col in two amino acids in repeats 9 and 10, which reduce RNA editing levels at site atp4-89 to about 50% in Ler. In a T-DNA insertion line, editing at this site is completely lost. In Vitis vinifera the gene most similar to MEF3 continues into a DYW extension beyond the common E domain. Complementation assays with various combinations of PPR and E domains from the vine and A. thaliana proteins show that the vine E region can substitute for the A. thaliana E region with or without the DYW domain. These findings suggest that the additional DYW domain does not disturb the MEF3 protein function in mitochondrial RNA editing in A. thaliana.
|
巻・号 |
53(2)
|
ページ |
358-67
|
公開日 |
2012-2-1
|
DOI |
10.1093/pcp/pcr182
|
PII |
pcr182
|
PMID |
22186180
|
MeSH |
Arabidopsis / genetics*
Arabidopsis / metabolism
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Base Sequence
Chromosome Mapping
DNA, Bacterial
Ecotype
Genetic Complementation Test
Mitochondria / genetics
Mitochondria / metabolism*
Molecular Sequence Data
Mutagenesis, Insertional
Protein Interaction Domains and Motifs
RNA Editing*
RNA, Plant / metabolism*
|
IF |
4.062
|
引用数 |
21
|
WOS 分野
|
PLANT SCIENCES
CELL BIOLOGY
|
リソース情報 |
シロイヌナズナ / 植物培養細胞・遺伝子 |
psh12413 |