RRC ID |
722
|
著者 |
Park KI, Choi JD, Hoshino A, Morita Y, Iida S.
|
タイトル |
An intragenic tandem duplication in a transcriptional regulatory gene for anthocyanin biosynthesis confers pale-colored flowers and seeds with fine spots in Ipomoea tricolor.
|
ジャーナル |
Plant J
|
Abstract |
While the wild-type morning glory (Ipomoea tricolor) displays bright-blue flowers and dark-brown seeds, its spontaneous mutant, Blue Star, carrying the mutable ivory seed-variegated (ivs-v) allele, exhibits pale-blue flowers with a few fine blue spots and ivory seeds with tiny dark-brown spots. The mutable allele is caused by an intragenic tandem duplication of 3.3 kbp within a gene for transcriptional activator containing a basic helix-loop-helix (bHLH) DNA-binding motif. Each of the tandem repeats is flanked by a 3-bp sequence AAT, indicating that the 3-bp microhomology is used to generate the tandem duplication. The transcripts in the pale-blue flower buds of the mutant contain an internal 583-bp tandem duplication that results in the production of a truncated polypeptide lacking the bHLH domain. The mRNA accumulation of most of the structural genes encoding enzymes for anthocyanin biosynthesis in the flower buds of the mutant was significantly reduced. The transcripts identical to the wild-type mRNAs for the transcriptional activator were present abundantly in blue spots of the variegated flowers, whereas the transcripts containing the 583-bp tandem duplication were predominant in the pale-blue background of the same flowers. The flower and seed variegations studied here are likely to be caused by somatic homologous recombination between an intragenic tandem duplication in the gene encoding a bHLH transcriptional activator for anthocyanin biosynthesis, whereas various flower variegations are reported to be caused by excision of DNA transposons inserted into pigmentation genes.
|
巻・号 |
38(5)
|
ページ |
840-9
|
公開日 |
2004-6-1
|
DOI |
10.1111/j.1365-313X.2004.02098.x
|
PII |
TPJ2098
|
PMID |
15144384
|
MeSH |
Anthocyanins / biosynthesis
Base Sequence
Cloning, Molecular
DNA Transposable Elements
DNA, Plant / genetics
Flowers / genetics*
Gene Expression Regulation, Plant / genetics*
Genome, Plant
Ipomoea / genetics*
Molecular Sequence Data
Pigmentation
RNA, Messenger / genetics
RNA, Plant / genetics
Seeds / genetics
Seeds / physiology
Transcription, Genetic / genetics*
|
IF |
6.141
|
引用数 |
36
|
WOS 分野
|
PLANT SCIENCES
|
リソース情報 |
アサガオ |
AK401
AK404 |