RRC ID |
11901
|
著者 |
Higuchi Y, Sage-Ono K, Sasaki R, Ohtsuki N, Hoshino A, Iida S, Kamada H, Ono M.
|
タイトル |
Constitutive expression of the GIGANTEA ortholog affects circadian rhythms and suppresses one-shot induction of flowering in Pharbitis nil, a typical short-day plant.
|
ジャーナル |
Plant Cell Physiol
|
Abstract |
GIGANTEA (GI) is a key regulator of flowering time, which is closely related to the circadian clock function in Arabidopsis. Mutations in the GI gene cause photoperiod-insensitive flowering and altered circadian rhythms. We isolated the GI ortholog PnGI from Pharbitis (Ipomoea) nil, an absolute short-day (SD) plant. PnGI mRNA expression showed diurnal rhythms that peaked at dusk under SD and long-day (LD) conditions, and also showed robust circadian rhythms under continuous dark (DD) and continuous light (LL) conditions. Short irradiation with red light during the flower-inductive dark period did not change PnGI expression levels, suggesting that such a night break does not abolish flowering by affecting the expression of PnGI. In Pharbitis, although a single dusk signal is sufficient to induce expression of the ortholog of FLOWERING LOCUS T (PnFT1), PnGI mRNA expression was not reset by single lights-off signals. Constitutive expression of PnGI (PnGI-OX) in transgenic plants altered period length in leaf-movement rhythms under LL and affected circadian rhythms of PnFT mRNA expression under DD. PnGI-OX plants formed fewer flower buds than the wild type when one-shot darkness was given. In PnGI-OX plants, expression of PnFT1 was down-regulated, suggesting that PnGI functions as a suppressor of flowering, possibly in part through down-regulation of PnFT1.
|
巻・号 |
52(4)
|
ページ |
638-50
|
公開日 |
2011-4-1
|
DOI |
10.1093/pcp/pcr023
|
PII |
pcr023
|
PMID |
21382978
|
MeSH |
Arabidopsis Proteins / genetics
Base Sequence
Circadian Rhythm / genetics*
Circadian Rhythm / radiation effects
DNA, Complementary / genetics
DNA, Plant / chemistry
DNA, Plant / genetics
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism
Darkness
Down-Regulation / genetics
Flowers / genetics
Flowers / metabolism
Flowers / physiology*
Gene Expression Regulation, Plant / radiation effects
Ipomoea nil / genetics
Ipomoea nil / growth & development
Ipomoea nil / physiology*
Ipomoea nil / radiation effects
Light
Molecular Sequence Data
Photoperiod
Plant Leaves / genetics
Plant Leaves / metabolism
Plant Proteins / genetics
Plant Proteins / metabolism*
Plants, Genetically Modified
RNA, Messenger / genetics
RNA, Plant / genetics
Sequence Analysis, DNA
Signal Transduction
|
IF |
4.062
|
引用数 |
22
|
WOS 分野
|
PLANT SCIENCES
CELL BIOLOGY
|
リソース情報 |
アサガオ |
EST clones |