RRC ID |
45651
|
著者 |
Vandamme J, Sidoli S, Mariani L, Friis C, Christensen J, Helin K, Jensen ON, Salcini AE.
|
タイトル |
H3K23me2 is a new heterochromatic mark in Caenorhabditis elegans.
|
ジャーナル |
Nucleic Acids Res
|
Abstract |
Genome-wide analyses in Caenorhabditis elegans show that post-translational modifications (PTMs) of histones are evolutionary conserved and distributed along functionally distinct genomic domains. However, a global profile of PTMs and their co-occurrence on the same histone tail has not been described in this organism. We used mass spectrometry based middle-down proteomics to analyze histone H3 N-terminal tails from C. elegans embryos for the presence, the relative abundance and the potential cross-talk of co-existing PTMs. This analysis highlighted that the lysine 23 of histone H3 (H3K23) is extensively modified by methylation and that tri-methylated H3K9 (H3K9me3) is exclusively detected on histone tails with di-methylated H3K23 (H3K23me2). Chromatin immunoprecipitation approaches revealed a positive correlation between H3K23me2 and repressive marks. By immunofluorescence analyses, H3K23me2 appears differentially regulated in germ and somatic cells, in part by the action of the histone demethylase JMJD-1.2. H3K23me2 is enriched in heterochromatic regions, localizing in H3K9me3 and heterochromatin protein like-1 (HPL-1)-positive foci. Biochemical analyses indicated that HPL-1 binds to H3K23me2 and interacts with a conserved CoREST repressive complex. Thus, our study suggests that H3K23me2 defines repressive domains and contributes to organizing the genome in distinct heterochromatic regions during embryogenesis.
|
巻・号 |
43(20)
|
ページ |
9694-710
|
公開日 |
2015-11-16
|
DOI |
10.1093/nar/gkv1063
|
PII |
gkv1063
|
PMID |
26476455
|
PMC |
PMC4787770
|
MeSH |
Acetylation
Animals
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / analysis
Caenorhabditis elegans Proteins / chemistry
Caenorhabditis elegans Proteins / metabolism*
Chromosomal Proteins, Non-Histone / analysis
Co-Repressor Proteins / metabolism
Embryo, Nonmammalian / metabolism
Embryonic Development
Germ Cells / metabolism
Heterochromatin / metabolism*
Histone Code*
Histone Demethylases / metabolism
Histones / analysis
Histones / chemistry
Histones / metabolism*
Lysine / metabolism
Methylation
Protein Processing, Post-Translational
|
IF |
11.502
|
引用数 |
18
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
線虫 |
tm3713
tm1489
tm4586 |