RRC ID |
41059
|
著者 |
Li C, Wang H, Jin P, Zheng W, Chu L, Liu C, Li J, Xiang W, Wang X.
|
タイトル |
Actinoallomurus bryophytorum sp. nov., an endophytic actinomycete isolated from moss (Bryophyta).
|
ジャーナル |
Antonie Van Leeuwenhoek
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Abstract |
A novel endophytic actinomycete, strain NEAU-TX1-15(T), was isolated from moss, collected from Wuchang, Heilongjiang province, north China. A polyphasic taxonomic study was carried out to establish the status of strain NEAU-TX1-15(T). Morphological and chemotaxonomic properties of strain NEAU-TX1-15(T) are consistent with the description of the genus Actinoallomurus. Strain NEAU-TX1-15(T) was observed to form short spiral or looped spore chains on aerial hyphae. The cell wall peptidoglycan was found to contain lysine and meso-diaminopimelic acid. The major menaquinones were identified as MK-9(H6) and MK-9(H8). The only phospholipid identified was phosphatidylglycerol. The major fatty acid was identified as iso-C16:0. Analysis of the 16S rRNA gene sequence supports the assignment of the novel strain to the genus Actinoallomurus, as it exhibits 99.2 % gene sequence similarity to that of Actinoallomurus yoronensis NBRC 103686(T). However, the low level of DNA-DNA relatedness allowed the strain to be differentiated from its close relative. Moreover, strain NEAU-TX1-15(T) could also be differentiated from A. yoronensis NBRC 103686(T) and other Actinoallomurus species showing high 16S rRNA gene sequence similarity (>98.0 %) by cultural and physiological characteristics. Therefore, the combination of phenotypic and chemotaxonomic data, and the DNA-DNA hybridization value, indicated that strain NEAU-TX1-15(T) represents a novel species of the genus Actinoallomurus for which the name Actinoallomurus bryophytorum sp. nov. is proposed. The type strain is NEAU-TX1-15(T) (=CGMCC 4.7200(T) = JCM 30340(T)).
|
巻・号 |
108(2)
|
ページ |
453-9
|
公開日 |
2015-8-1
|
DOI |
10.1007/s10482-015-0498-z
|
PMID |
26033369
|
MeSH |
Actinobacteria / classification*
Actinobacteria / genetics
Actinobacteria / isolation & purification*
Actinobacteria / physiology
Bacterial Typing Techniques
Bryophyta / microbiology*
Cell Wall / chemistry
China
Cluster Analysis
Cytosol / chemistry
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Diaminopimelic Acid / analysis
Endophytes / classification*
Endophytes / genetics
Endophytes / isolation & purification*
Endophytes / physiology
Fatty Acids / analysis
Microscopy, Electron, Scanning
Molecular Sequence Data
Nucleic Acid Hybridization
Peptidoglycan / analysis
Phospholipids / analysis
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Spores, Bacterial / cytology
Vitamin K 2 / analysis
|
IF |
2.674
|
引用数 |
1
|
WOS 分野
|
MICROBIOLOGY
|
リソース情報 |
一般微生物 |
JCM 30340 |