RRC ID |
36751
|
著者 |
Mochimaru Y, Azuma M, Oshima N, Ichijo Y, Satou K, Matsuda K, Asaoka Y, Nishina H, Nakakura T, Mogi C, Sato K, Okajima F, Tomura H.
|
タイトル |
Extracellular acidification activates ovarian cancer G-protein-coupled receptor 1 and GPR4 homologs of zebra fish.
|
ジャーナル |
Biochem Biophys Res Commun
|
Abstract |
Mammalian ovarian G-protein-coupled receptor 1 (OGR1) and GPR4 are identified as a proton-sensing G-protein-coupled receptor coupling to multiple intracellular signaling pathways. In the present study, we examined whether zebra fish OGR1 and GPR4 homologs (zOGR1 and zGPR4) could sense protons and activate the multiple intracellular signaling pathways and, if so, whether the similar positions of histidine residue, which is critical for sensing protons in mammalian OGR and GPR4, also play a role to sense protons and activate the multiple signaling pathways in the zebra fish receptors. We found that extracellular acidic pH stimulated CRE-, SRE-, and NFAT-promoter activities in zOGR1 overexpressed cells and stimulated CRE- and SRE- but not NFAT-promoter activities in zGPR4 overexpressed cells. The substitution of histidine residues at the 12th, 15th, 162th, and 264th positions from the N-terminal of zOGR1 with phenylalanine attenuated the proton-induced SRE-promoter activities. The mutation of the histidine residue at the 78th but not the 84th position from the N-terminal of zGPR4 to phenylalanine attenuated the proton-induced SRE-promoter activities. These results suggest that zOGR1 and zGPR4 are also proton-sensing G-protein-coupled receptors, and the receptor activation mechanisms may be similar to those of the mammalian receptors.
|
巻・号 |
457(4)
|
ページ |
493-9
|
公開日 |
2015-2-20
|
DOI |
10.1016/j.bbrc.2014.12.105
|
PII |
S0006-291X(14)02319-5
|
PMID |
25576873
|
MeSH |
Amino Acid Sequence
Animals
Gene Expression Regulation
HEK293 Cells
Humans
Hydrogen-Ion Concentration
Molecular Sequence Data
Protons*
Receptors, G-Protein-Coupled / chemistry
Receptors, G-Protein-Coupled / genetics
Receptors, G-Protein-Coupled / metabolism*
Sequence Alignment
Signal Transduction
Zebrafish / genetics
Zebrafish / metabolism*
Zebrafish Proteins / chemistry
Zebrafish Proteins / genetics
Zebrafish Proteins / metabolism*
|
IF |
2.985
|
引用数 |
8
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
293T(RCB2202) |