RRC ID |
48190
|
著者 |
Lee S, Chung JM, Yun HJ, Won J, Jung HS.
|
タイトル |
New insight into multifunctional role of peroxiredoxin family protein: Determination of DNA protection properties of bacterioferritin comigratory protein under hyperthermal and oxidative stresses.
|
ジャーナル |
Biochem Biophys Res Commun
|
Abstract |
Bacterioferritin comigratory protein (BCP) is a monomeric conformer acting as a putative thiol-dependent bacterial peroxidase, however molecular basis of DNA-protection via DNA-binding has not been clearly understood. In this study, we characterized the DNA binding properties of BCP using various lengths and differently shaped architectures of DNA. An electrophoretic mobility shift assay and electron microscopy analysis showed that recombinant TkBCP bound to DNA of a circular shape (double-stranded DNA and single-stranded DNA) and a linear shape (16-1000 bp) as well as various architectures of DNA. In addition, DNA protection experiments indicated that TkBCP can protect DNA against hyperthermal and oxidative stress by removing highly reactive oxygen species (ROS) or by protecting DNA from thermal degradation. Based on these results, we suggest that TkBCP is a multi-functional DNA-binding protein which has DNA chaperon and antioxidant functions.
|
巻・号 |
469(4)
|
ページ |
1028-33
|
公開日 |
2016-1-22
|
DOI |
10.1016/j.bbrc.2015.12.099
|
PII |
S0006-291X(15)31104-9
|
PMID |
26723255
|
MeSH |
Bacterial Proteins / chemistry*
Binding Sites
DNA / chemistry*
DNA / genetics*
DNA Damage*
Heat-Shock Response
Hot Temperature
Oxidative Stress
Protein Binding
Reactive Oxygen Species / chemistry*
Temperature
|
IF |
2.985
|
引用数 |
7
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
一般微生物 |
JCM 12380 |