RRC ID |
47965
|
著者 |
Yu T, Graf M, Renn J, Schartl M, Larionova D, Huysseune A, Witten PE, Winkler C.
|
タイトル |
A vertebrate-specific and essential role for osterix in osteogenesis revealed by gene knockout in the teleost medaka.
|
ジャーナル |
Development
|
Abstract |
osterix (osx; sp7) encodes a zinc-finger transcription factor that controls osteoblast differentiation in mammals. Although identified in all vertebrate lineages, its role in non-mammalian bone formation remains elusive. Here, we show that an osx mutation in medaka results in severe bone defects and larval lethality. Pre-osteoblasts fail to differentiate leading to severe intramembranous and perichondral ossification defects. The notochord sheath mineralizes normally, supporting the idea of an osteoblast-independent mechanism for teleost vertebral centra formation. This study establishes a key role for Osx for bone formation in a non-mammalian species, and reveals conserved and non-conserved features in vertebrate bone formation.
|
巻・号 |
144(2)
|
ページ |
265-271
|
公開日 |
2017-1-15
|
DOI |
10.1242/dev.139550
|
PII |
dev.139550
|
PMID |
27993982
|
MeSH |
Animals
Animals, Genetically Modified
Calcification, Physiologic / genetics
Gene Expression Regulation, Developmental
Gene Knockout Techniques
Notochord / embryology
Oryzias / embryology*
Oryzias / genetics*
Osteogenesis / genetics*
Phylogeny
Sp7 Transcription Factor
Species Specificity
Transcription Factors / genetics
Transcription Factors / physiology*
Vertebrates / embryology
Vertebrates / genetics
Zebrafish Proteins / physiology
|
IF |
5.611
|
引用数 |
17
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
|
リソース情報 |
メダカ |
osx_tv1mutant/osx:mCherry (TG1138)
col10a1:nlGFP (TG1129) |