RRC ID |
11423
|
著者 |
Kubo T, Yamagata Y, Eguchi M, Yoshimura A.
|
タイトル |
A novel epistatic interaction at two loci causing hybrid male sterility in an inter-subspecific cross of rice (Oryza sativa L.).
|
ジャーナル |
Genes Genet Syst
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Abstract |
Postzygotic reproductive isolation (RI) often arises in inter-subspecific crosses as well as inter-specific crosses of rice (Oryza sativa L.). To further understand the genetic architecture of the postzygotic RI, we analyzed genes causing hybrid sterility and hybrid breakdown in a rice inter-subspecific cross. Here we report hybrid male sterility caused by epistatic interaction between two novel genes, S24 and S35, which were identified on rice chromosomes 5 and 1, respectively. Genetic analysis using near-isogenic lines (NILs) carrying IR24 (ssp. indica) segments with Asominori (ssp. japonica) genetic background revealed a complicated aspect of the epistasis. Allelic interaction at the S24 locus in the heterozygous plants caused abortion of male gametes carrying the Asominori allele (S24-as) independent of the S35 genotype. On the other hand, male gametes carrying the Asominori allele at the S35 locus (S35-as) showed abortion only when the IR24 allele at the S24 locus (S24-ir) was concurrently introgressed into the S35 heterozygous plants, indicating that the sterility phenotype due to S35 was dependent on the S24 genotype through negative epistasis between S24-ir and S35-as alleles. Due to the interaction between S24 and S35, self-pollination of the double heterozygous plants produced pollen-sterile progeny carrying the S24-ir/S24-ir S35-as/S35-ir genotype in addition to the S24 heterozygous plants. This result suggests that the S35 gene might function as a modifier of S24. This study presents strong evidence for the importance of epistatic interaction as a part of the genetic architecture of hybrid sterility in rice. In addition, it suggests that diverse systems have been developed as postzygotic RI mechanisms within the rice.
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巻・号 |
83(6)
|
ページ |
443-53
|
公開日 |
2008-12-1
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DOI |
10.1266/ggs.83.443
|
PII |
JST.JSTAGE/ggs/83.443
|
PMID |
19282622
|
MeSH |
Chromosome Mapping
Crosses, Genetic
Epistasis, Genetic*
Genes, Plant / genetics*
Hybridization, Genetic*
Infertility / genetics*
Oryza / genetics*
Phenotype
Polymorphism, Restriction Fragment Length
Reproduction / genetics
Species Specificity
|
IF |
0.917
|
引用数 |
33
|
WOS 分野
|
GENETICS & HEREDITY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
イネ |
CSSL |