| RRC ID |
33603
|
| 著者 |
Miyazaki T, Ichikawa M, Iino H, Nishikawa A, Tonozuka T.
|
| タイトル |
Crystal structure and substrate-binding mode of GH63 mannosylglycerate hydrolase from Thermus thermophilus HB8.
|
| ジャーナル |
J Struct Biol
|
| Abstract |
Glycoside hydrolase family 63 (GH63) proteins are found in eukaryotes such as processing α-glucosidase I and also many bacteria and archaea. Recent studies have identified two bacterial and one plant GH63 mannosylglycerate hydrolases that act on both glucosylglycerate and mannosylglycerate, which are compatible solutes found in many thermophilic prokaryotes and some plants. Here we report the 1.67-Å crystal structure of one of these GH63 mannosylglycerate hydrolases, Tt8MGH from Thermus thermophilus HB8, which is 99% homologous to mannosylglycerate hydrolase from T. thermophilus HB27. Tt8MGH consists of a single (α/α)6-barrel catalytic domain with two additional helices and two long loops which form a homotrimer. The structures of this protein in complexes with glucose or glycerate were also determined at 1.77- or 2.10-Å resolution, respectively. A comparison of these structures revealed that the conformations of three flexible loops were largely different from each other. The conformational changes may be induced by ligand binding and serve to form finger-like structures for holding substrates. These findings represent the first-ever proposed substrate recognition mechanism for GH63 mannosylglycerate hydrolase.
|
| 巻・号 |
190(1)
|
| ページ |
21-30
|
| 公開日 |
2015-4-1
|
| DOI |
10.1016/j.jsb.2015.02.006
|
| PII |
S1047-8477(15)00037-4
|
| PMID |
25712767
|
| MeSH |
Amino Acid Sequence
Bacterial Proteins / chemistry*
Catalytic Domain
Crystallography, X-Ray
Glyceric Acids / chemistry
Glycoside Hydrolases / chemistry*
Hydrogen Bonding
Mannose / analogs & derivatives
Mannose / chemistry
Models, Molecular
Molecular Sequence Data
Phylogeny
Protein Binding
Protein Structure, Quaternary
Protein Structure, Secondary
Sequence Alignment
Structural Homology, Protein
Substrate Specificity
Thermus thermophilus / enzymology*
|
| IF |
3.071
|
| 引用数 |
7
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 遺伝子材料 |
Thermus thermophilus expression plasmid TEx11B04 (THR004428) |
